Evaluation of viability and proliferative activity of human urothelial cells cultured onto xenogenic tissue-engineered extracellular matrices.

Hdl Handle:
http://hdl.handle.net/10147/135764
Title:
Evaluation of viability and proliferative activity of human urothelial cells cultured onto xenogenic tissue-engineered extracellular matrices.
Authors:
Davis, Niall F; Callanan, Anthony; McGuire, Barry B; Flood, Hugh D; McGloughlin, Tim M
Affiliation:
Department of Urology, Mid-Western Regional Hospital, Limerick, Ireland.
Citation:
Evaluation of viability and proliferative activity of human urothelial cells cultured onto xenogenic tissue-engineered extracellular matrices. 2011, 77 (4):1007.e1-7 Urology
Journal:
Urology
Issue Date:
Apr-2011
URI:
http://hdl.handle.net/10147/135764
DOI:
10.1016/j.urology.2010.11.036
PubMed ID:
21256541
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/21256541
Abstract:
To evaluate the viability and proliferative activity of human urothelial cells (HUCs) cultured on tissue-engineered extracellular matrix scaffolds and to assess the potential of extracellular matrixes to support the growth of HUCs in their expected in vivo urine environment.; HUCs were obtained by bladder biopsy and cultured onto the luminal and abluminal surfaces of decellularized porcine small intestinal submucosa (SIS) and porcine urinary bladder matrix (UBM). In addition, HUCs were cultured in optimal in vitro growth conditions and in their expected in vivo urine environment. The attachment, viability, and proliferative activity of HUCs were evaluated and compared using quantitative viability indicators and fluorescent markers for intracellular esterase activity and plasma membrane integrity.; The luminal and abluminal surfaces of the UBM demonstrated significantly greater HUC viability and proliferative activity compared with the luminal and abluminal surfaces of the SIS grafts (P < .0001). Culture of HUCs in a simulated in vivo urine environment significantly affected cell viability (P < .0001). Proliferative activity was immeasurable on cell-seeded scaffolds that were cultured in a urine environment after 48 hours of growth (P < .0001).; This is the first comparative report of UBM and SIS. Our results have demonstrated that UBM has significantly greater regenerative potential for HUCs compared with SIS. However, the perceived potential for extracellular matrixes in reconstructive urology might be limited by their inability to induce urothelial regeneration in a urine environment.
Item Type:
Article
Language:
en
MeSH:
Cell Culture Techniques; Cell Proliferation; Cell Survival; Cells, Cultured; Esterases; Ethidium; Extracellular Matrix; Humans; Immunohistochemistry; Intercalating Agents; Intestinal Mucosa; Muscle, Smooth; Oxazines; Tissue Engineering; Tissue Scaffolds; Transplantation, Heterologous; Urothelium; Xanthenes
ISSN:
1527-9995

Full metadata record

DC FieldValue Language
dc.contributor.authorDavis, Niall Fen
dc.contributor.authorCallanan, Anthonyen
dc.contributor.authorMcGuire, Barry Ben
dc.contributor.authorFlood, Hugh Den
dc.contributor.authorMcGloughlin, Tim Men
dc.date.accessioned2011-07-11T13:52:19Z-
dc.date.available2011-07-11T13:52:19Z-
dc.date.issued2011-04-
dc.identifier.citationEvaluation of viability and proliferative activity of human urothelial cells cultured onto xenogenic tissue-engineered extracellular matrices. 2011, 77 (4):1007.e1-7 Urologyen
dc.identifier.issn1527-9995-
dc.identifier.pmid21256541-
dc.identifier.doi10.1016/j.urology.2010.11.036-
dc.identifier.urihttp://hdl.handle.net/10147/135764-
dc.description.abstractTo evaluate the viability and proliferative activity of human urothelial cells (HUCs) cultured on tissue-engineered extracellular matrix scaffolds and to assess the potential of extracellular matrixes to support the growth of HUCs in their expected in vivo urine environment.-
dc.description.abstractHUCs were obtained by bladder biopsy and cultured onto the luminal and abluminal surfaces of decellularized porcine small intestinal submucosa (SIS) and porcine urinary bladder matrix (UBM). In addition, HUCs were cultured in optimal in vitro growth conditions and in their expected in vivo urine environment. The attachment, viability, and proliferative activity of HUCs were evaluated and compared using quantitative viability indicators and fluorescent markers for intracellular esterase activity and plasma membrane integrity.-
dc.description.abstractThe luminal and abluminal surfaces of the UBM demonstrated significantly greater HUC viability and proliferative activity compared with the luminal and abluminal surfaces of the SIS grafts (P < .0001). Culture of HUCs in a simulated in vivo urine environment significantly affected cell viability (P < .0001). Proliferative activity was immeasurable on cell-seeded scaffolds that were cultured in a urine environment after 48 hours of growth (P < .0001).-
dc.description.abstractThis is the first comparative report of UBM and SIS. Our results have demonstrated that UBM has significantly greater regenerative potential for HUCs compared with SIS. However, the perceived potential for extracellular matrixes in reconstructive urology might be limited by their inability to induce urothelial regeneration in a urine environment.-
dc.language.isoenen
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/21256541en
dc.subject.meshCell Culture Techniques-
dc.subject.meshCell Proliferation-
dc.subject.meshCell Survival-
dc.subject.meshCells, Cultured-
dc.subject.meshEsterases-
dc.subject.meshEthidium-
dc.subject.meshExtracellular Matrix-
dc.subject.meshHumans-
dc.subject.meshImmunohistochemistry-
dc.subject.meshIntercalating Agents-
dc.subject.meshIntestinal Mucosa-
dc.subject.meshMuscle, Smooth-
dc.subject.meshOxazines-
dc.subject.meshTissue Engineering-
dc.subject.meshTissue Scaffolds-
dc.subject.meshTransplantation, Heterologous-
dc.subject.meshUrothelium-
dc.subject.meshXanthenes-
dc.titleEvaluation of viability and proliferative activity of human urothelial cells cultured onto xenogenic tissue-engineered extracellular matrices.en
dc.typeArticleen
dc.contributor.departmentDepartment of Urology, Mid-Western Regional Hospital, Limerick, Ireland.en
dc.identifier.journalUrologyen
dc.description.provinceMunster-

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