Porphyrin-magnetite nanoconjugates for biological imaging

Hdl Handle:
http://hdl.handle.net/10147/129334
Title:
Porphyrin-magnetite nanoconjugates for biological imaging
Authors:
Nowostawska, Malgorzata; Corr, Serena A; Byrne, Stephen J; Conroy, Jennifer; Volkov, Yuri; Gun'ko, Yurii K
Issue Date:
8-Apr-2011
URI:
http://hdl.handle.net/10147/129334
Abstract:
Abstract Background The use of silica coated magnetic nanoparticles as contrast agents has resulted in the production of highly stable, non-toxic solutions that can be manipulated via an external magnetic field. As a result, the interaction of these nanocomposites with cells is of vital importance in understanding their behaviour and biocompatibility. Here we report the preparation, characterisation and potential application of new "two-in-one" magnetic fluorescent nanocomposites composed of silica-coated magnetite nanoparticles covalently linked to a porphyrin moiety. Method The experiments were performed by administering porphyrin functionalised silica-coated magnetite nanoparticles to THP-1 cells, a human acute monocytic leukaemia cell line. Cells were cultured in RPMI 1640 medium with 25 mM HEPES supplemented with heat-inactivated foetal bovine serum (FBS). Results We have synthesised, characterised and analysed in vitro, a new multimodal (magnetic and fluorescent) porphyrin magnetic nanoparticle composite (PMNC). Initial co-incubation experiments performed with THP-1 macrophage cells were promising; however the PMNC photobleached under confocal microscopy study. β-mercaptoethanol (β-ME) was employed to counteract this problem and resulted not only in enhanced fluorescence emission, but also allowed for elongated imaging and increased exposure times of the PMNC in a cellular environment. Conclusion Our experiments have demonstrated that β-ME visibly enhances the emission intensity. No deleterious effects to the cells were witnessed upon co-incubation with β-ME alone and no increases in background fluorescence were recorded. These results should present an interest for further development of in vitro biological imaging techniques.
ISSN:
http://dx.doi.org/10.1186/1477-3155-9-13

Full metadata record

DC FieldValue Language
dc.contributor.authorNowostawska, Malgorzataen
dc.contributor.authorCorr, Serena Aen
dc.contributor.authorByrne, Stephen Jen
dc.contributor.authorConroy, Jenniferen
dc.contributor.authorVolkov, Yurien
dc.contributor.authorGun'ko, Yurii Ken
dc.date.accessioned2011-05-10T11:09:13Z-
dc.date.available2011-05-10T11:09:13Z-
dc.date.issued2011-04-08-
dc.identifier.issnhttp://dx.doi.org/10.1186/1477-3155-9-13-
dc.identifier.urihttp://hdl.handle.net/10147/129334-
dc.description.abstractAbstract Background The use of silica coated magnetic nanoparticles as contrast agents has resulted in the production of highly stable, non-toxic solutions that can be manipulated via an external magnetic field. As a result, the interaction of these nanocomposites with cells is of vital importance in understanding their behaviour and biocompatibility. Here we report the preparation, characterisation and potential application of new "two-in-one" magnetic fluorescent nanocomposites composed of silica-coated magnetite nanoparticles covalently linked to a porphyrin moiety. Method The experiments were performed by administering porphyrin functionalised silica-coated magnetite nanoparticles to THP-1 cells, a human acute monocytic leukaemia cell line. Cells were cultured in RPMI 1640 medium with 25 mM HEPES supplemented with heat-inactivated foetal bovine serum (FBS). Results We have synthesised, characterised and analysed in vitro, a new multimodal (magnetic and fluorescent) porphyrin magnetic nanoparticle composite (PMNC). Initial co-incubation experiments performed with THP-1 macrophage cells were promising; however the PMNC photobleached under confocal microscopy study. β-mercaptoethanol (β-ME) was employed to counteract this problem and resulted not only in enhanced fluorescence emission, but also allowed for elongated imaging and increased exposure times of the PMNC in a cellular environment. Conclusion Our experiments have demonstrated that β-ME visibly enhances the emission intensity. No deleterious effects to the cells were witnessed upon co-incubation with β-ME alone and no increases in background fluorescence were recorded. These results should present an interest for further development of in vitro biological imaging techniques.-
dc.titlePorphyrin-magnetite nanoconjugates for biological imagingen
dc.language.rfc3066en-
dc.rights.holderNowostawska et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2011-05-09T16:04:23Z-
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