Platelet function in the first trimester [Poster]

Hdl Handle:
http://hdl.handle.net/10147/128798
Title:
Platelet function in the first trimester [Poster]
Authors:
Flood, Karen; Peace, Aaron; Kent, Etaoin; Tedesco, Tony; Dicker, P.; Geary, Michael; Kenny, Dermot; Malone, Fergal D
Affiliation:
Rotunda Hospital
Publisher:
Mosby, Inc.
Journal:
American Journal of Obstetrics and Gynaecology
Issue Date:
Dec-2009
URI:
http://hdl.handle.net/10147/128798
Additional Links:
http://www.ajog.org/article/S0002-9378(09)01336-2/fulltext
Item Type:
Presentation
Language:
en
Description:
OBJECTIVE: To determine if platelet reactivity is altered in the first trimester of pregnancy. STUDY DESIGN: 10 healthy pregnant volunteers were recruited at time of first trimester screening (between 11-13 weeks gestation). Controls included 10 age-matched healthy subjects with prior good obstetric outcome. A 30 ml blood sample was taken and platelet function assessed using a novel assay of platelet reactivity. Platelet aggregation to multiple concentrations of Arachidonic Acid (AA), Collagen (Col),Epinephrine (Epi) and Adenosine-Diphosphate (ADP) were measured simultaneously over successive time-points using a modification of light transmission aggregometry. Dose–response curves were graphed with the maximum aggregatory response calculated to multiple doses of each agonist. RESULTS: The pregnant group were significantly less reactive to all submaximal doses of Epi:1%versus25%at dose 0.78 mcM,5%versus 40% at dose 3.125mcM, 15% versus 50% at dose 12.5 mcM, 38% versus 65% at dose50mcMand 60% versus 76% at dose 200mcM with overall paired p value 0.01. The pregnant group were also less reactive to submaximal doses of AA: 11% versus 20% at dose 0.93mg/ml and 14% versus 35% at dose 1.875mg/ml (p 0.06) and submaximal doses of Coll: 48% versus 72% at dose 0.71 mg/ml and 68% versus 82% at dose 1.43mg/ml (p 0.06). The groups were similar in their aggregatory reponse to ADP. CONCLUSION: Increased platelet reactivity in pregnancy has been shown in numerous in vivo and in vitro studies, however, this effect has usually been demonstrated in the second and third trimesters. Using a novel platelet assay this study has demonstrated global platelet under-reactivity early in pregancy which may represent an adaptive mechanism to allow successful implantation. Further studies are planned to evaluate the outcome of pregancies that do not follow this normal platelet functional response.

Full metadata record

DC FieldValue Language
dc.contributor.authorFlood, Karenen
dc.contributor.authorPeace, Aaronen
dc.contributor.authorKent, Etaoinen
dc.contributor.authorTedesco, Tonyen
dc.contributor.authorDicker, P.en
dc.contributor.authorGeary, Michaelen
dc.contributor.authorKenny, Dermoten
dc.contributor.authorMalone, Fergal Den
dc.date.accessioned2011-04-27T15:10:55Z-
dc.date.available2011-04-27T15:10:55Z-
dc.date.issued2009-12-
dc.identifier.urihttp://hdl.handle.net/10147/128798-
dc.descriptionOBJECTIVE: To determine if platelet reactivity is altered in the first trimester of pregnancy. STUDY DESIGN: 10 healthy pregnant volunteers were recruited at time of first trimester screening (between 11-13 weeks gestation). Controls included 10 age-matched healthy subjects with prior good obstetric outcome. A 30 ml blood sample was taken and platelet function assessed using a novel assay of platelet reactivity. Platelet aggregation to multiple concentrations of Arachidonic Acid (AA), Collagen (Col),Epinephrine (Epi) and Adenosine-Diphosphate (ADP) were measured simultaneously over successive time-points using a modification of light transmission aggregometry. Dose–response curves were graphed with the maximum aggregatory response calculated to multiple doses of each agonist. RESULTS: The pregnant group were significantly less reactive to all submaximal doses of Epi:1%versus25%at dose 0.78 mcM,5%versus 40% at dose 3.125mcM, 15% versus 50% at dose 12.5 mcM, 38% versus 65% at dose50mcMand 60% versus 76% at dose 200mcM with overall paired p value 0.01. The pregnant group were also less reactive to submaximal doses of AA: 11% versus 20% at dose 0.93mg/ml and 14% versus 35% at dose 1.875mg/ml (p 0.06) and submaximal doses of Coll: 48% versus 72% at dose 0.71 mg/ml and 68% versus 82% at dose 1.43mg/ml (p 0.06). The groups were similar in their aggregatory reponse to ADP. CONCLUSION: Increased platelet reactivity in pregnancy has been shown in numerous in vivo and in vitro studies, however, this effect has usually been demonstrated in the second and third trimesters. Using a novel platelet assay this study has demonstrated global platelet under-reactivity early in pregancy which may represent an adaptive mechanism to allow successful implantation. Further studies are planned to evaluate the outcome of pregancies that do not follow this normal platelet functional response.en
dc.language.isoenen
dc.publisherMosby, Inc.en
dc.relation.urlhttp://www.ajog.org/article/S0002-9378(09)01336-2/fulltexten
dc.titlePlatelet function in the first trimester [Poster]en
dc.typePresentationen
dc.contributor.departmentRotunda Hospitalen
dc.identifier.journalAmerican Journal of Obstetrics and Gynaecologyen
dc.description.provinceLeinster-
All Items in Lenus, The Irish Health Repository are protected by copyright, with all rights reserved, unless otherwise indicated.