Neurofilament subunit (NFL) head domain phosphorylation regulates axonal transport of neurofilaments.

Hdl Handle:
http://hdl.handle.net/10147/128719
Title:
Neurofilament subunit (NFL) head domain phosphorylation regulates axonal transport of neurofilaments.
Authors:
Yates, Darran M; Manser, Catherine; De Vos, Kurt J; Shaw, Christopher E; McLoughlin, Declan M; Miller, Christopher C J
Affiliation:
MRC Centre for Neurodegeneration Research, Department of Neuroscience P037, Institute of Psychiatry, King's College London, De Crespigny Park, Denmark Hill, London SE58AF, UK.
Citation:
Neurofilament subunit (NFL) head domain phosphorylation regulates axonal transport of neurofilaments. 2009, 88 (4):193-202 Eur. J. Cell Biol.
Journal:
European journal of cell biology
Issue Date:
Apr-2009
URI:
http://hdl.handle.net/10147/128719
DOI:
10.1016/j.ejcb.2008.11.004
PubMed ID:
19147253
Additional Links:
doi:10.1016/j.ejcb.2008.11.004
Abstract:
Neurofilaments are the intermediate filaments of neurons and are synthesised in neuronal cell bodies and then transported through axons. Neurofilament light chain (NFL) is a principal component of neurofilaments, and phosphorylation of NFL head domain is believed to regulate the assembly of neurofilaments. However, the role that NFL phosphorylation has on transport of neurofilaments is poorly understood. To address this issue, we monitored axonal transport of phosphorylation mutants of NFL. We mutated four known phosphorylation sites in NFL head domain to either preclude phosphorylation, or mimic permanent phosphorylation. Mutation to preclude phosphorylation had no effect on transport but mutation of three sites to mimic permanent phosphorylation inhibited transport. Mutation of all four sites together to mimic permanent phosphorylation proved especially potent at inhibiting transport and also disrupted neurofilament assembly. Our results suggest that NFL head domain phosphorylation is a regulator of neurofilament axonal transport.
Item Type:
Article
Language:
en
MeSH:
Animals; Axonal Transport; Axons; Cells, Cultured; Intermediate Filaments; Mutant Proteins; Neurons; Phosphorylation; Rats; Transfection
ISSN:
1618-1298

Full metadata record

DC FieldValue Language
dc.contributor.authorYates, Darran Men
dc.contributor.authorManser, Catherineen
dc.contributor.authorDe Vos, Kurt Jen
dc.contributor.authorShaw, Christopher Een
dc.contributor.authorMcLoughlin, Declan Men
dc.contributor.authorMiller, Christopher C Jen
dc.date.accessioned2011-04-27T09:31:46Z-
dc.date.available2011-04-27T09:31:46Z-
dc.date.issued2009-04-
dc.identifier.citationNeurofilament subunit (NFL) head domain phosphorylation regulates axonal transport of neurofilaments. 2009, 88 (4):193-202 Eur. J. Cell Biol.en
dc.identifier.issn1618-1298-
dc.identifier.pmid19147253-
dc.identifier.doi10.1016/j.ejcb.2008.11.004-
dc.identifier.urihttp://hdl.handle.net/10147/128719-
dc.description.abstractNeurofilaments are the intermediate filaments of neurons and are synthesised in neuronal cell bodies and then transported through axons. Neurofilament light chain (NFL) is a principal component of neurofilaments, and phosphorylation of NFL head domain is believed to regulate the assembly of neurofilaments. However, the role that NFL phosphorylation has on transport of neurofilaments is poorly understood. To address this issue, we monitored axonal transport of phosphorylation mutants of NFL. We mutated four known phosphorylation sites in NFL head domain to either preclude phosphorylation, or mimic permanent phosphorylation. Mutation to preclude phosphorylation had no effect on transport but mutation of three sites to mimic permanent phosphorylation inhibited transport. Mutation of all four sites together to mimic permanent phosphorylation proved especially potent at inhibiting transport and also disrupted neurofilament assembly. Our results suggest that NFL head domain phosphorylation is a regulator of neurofilament axonal transport.-
dc.language.isoenen
dc.relation.urldoi:10.1016/j.ejcb.2008.11.004en
dc.subject.meshAnimals-
dc.subject.meshAxonal Transport-
dc.subject.meshAxons-
dc.subject.meshCells, Cultured-
dc.subject.meshIntermediate Filaments-
dc.subject.meshMutant Proteins-
dc.subject.meshNeurons-
dc.subject.meshPhosphorylation-
dc.subject.meshRats-
dc.subject.meshTransfection-
dc.titleNeurofilament subunit (NFL) head domain phosphorylation regulates axonal transport of neurofilaments.en
dc.typeArticleen
dc.contributor.departmentMRC Centre for Neurodegeneration Research, Department of Neuroscience P037, Institute of Psychiatry, King's College London, De Crespigny Park, Denmark Hill, London SE58AF, UK.en
dc.identifier.journalEuropean journal of cell biologyen
dc.description.provinceLeinster-

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