Intravenous glutamine enhances COX-2 activity giving cardioprotection.

Hdl Handle:
http://hdl.handle.net/10147/127682
Title:
Intravenous glutamine enhances COX-2 activity giving cardioprotection.
Authors:
McGuinness, Jonathan; Neilan, Tom G; Cummins, Rob; Sharkasi, Adel; Bouchier-Hayes, David; Redmond, J Mark
Affiliation:
The Royal College of Surgeons in Ireland, Department of Surgery, Beaumont Hospital, Dublin, Ireland.
Citation:
Intravenous glutamine enhances COX-2 activity giving cardioprotection. 2009, 152 (1):140-7 J. Surg. Res.
Journal:
The Journal of surgical research
Issue Date:
Mar-2009
URI:
http://hdl.handle.net/10147/127682
DOI:
10.1016/j.jss.2008.03.045
PubMed ID:
18708191
Abstract:
Preconditioning, a highly evolutionary conserved endogenous protective response, provides the most powerful form of anti-infarct protection known. We investigated whether acute intravenous glutamine, through an effect on cyclooxygenase (COX)-2 and heat shock protein (HSP) 72, might induce preconditioning.; Male New Zealand white rabbits (n = 28) received either 0.5 g/kg glutamine in 0.9% saline or saline only in divided doses for 3 d. The large marginal branch of the left coronary was occluded for 30 min; cardiac function was assessed during 3 h of reperfusion, and infarct size was measured. 6-Keto-PGF-1alpha, nitrate, and malonaldehyde serum levels were determined. Hearts were taken from a further group of pretreated rabbits (n = 10) to assess myocardial COX-2 and HSP72 levels.; Glutamine pretreatment resulted in a 39% reduction in infarct size (30.7 +/- 2.0% versus 50.4 +/- 2.1% controls; P < 0.01). Myocardial COX-2 levels were significantly elevated with pretreatment (P < 0.05) and were mirrored by higher serum 6-keto-PGF-1alpha levels prior to ischemia (69 +/- 13 versus 18 +/- 21 pg/mL in controls, P = 0.027). There was no significant difference in myocardial HSP72 or serum nitrate levels following pretreatment, or malonaldehyde levels during reperfusion.; Glutamine pretreatment confers anti-infarct protection through up-regulation of COX-2, a key mediator of delayed preconditioning protection. Previous confirmation of its clinical safety profile at these doses suggests an acceptable strategy for inducing preconditioning for perioperative protection.
Item Type:
Article
Language:
en
MeSH:
6-Ketoprostaglandin F1 alpha; Animals; Cyclooxygenase 2; Glutamine; HSP72 Heat-Shock Proteins; Hemodynamics; Injections, Intravenous; Ischemic Preconditioning, Myocardial; Male; Malondialdehyde; Myocardial Reperfusion Injury; Myocardium; Nitrates; Rabbits
ISSN:
1095-8673

Full metadata record

DC FieldValue Language
dc.contributor.authorMcGuinness, Jonathanen
dc.contributor.authorNeilan, Tom Gen
dc.contributor.authorCummins, Roben
dc.contributor.authorSharkasi, Adelen
dc.contributor.authorBouchier-Hayes, Daviden
dc.contributor.authorRedmond, J Marken
dc.date.accessioned2011-04-07T10:59:14Z-
dc.date.available2011-04-07T10:59:14Z-
dc.date.issued2009-03-
dc.identifier.citationIntravenous glutamine enhances COX-2 activity giving cardioprotection. 2009, 152 (1):140-7 J. Surg. Res.en
dc.identifier.issn1095-8673-
dc.identifier.pmid18708191-
dc.identifier.doi10.1016/j.jss.2008.03.045-
dc.identifier.urihttp://hdl.handle.net/10147/127682-
dc.description.abstractPreconditioning, a highly evolutionary conserved endogenous protective response, provides the most powerful form of anti-infarct protection known. We investigated whether acute intravenous glutamine, through an effect on cyclooxygenase (COX)-2 and heat shock protein (HSP) 72, might induce preconditioning.-
dc.description.abstractMale New Zealand white rabbits (n = 28) received either 0.5 g/kg glutamine in 0.9% saline or saline only in divided doses for 3 d. The large marginal branch of the left coronary was occluded for 30 min; cardiac function was assessed during 3 h of reperfusion, and infarct size was measured. 6-Keto-PGF-1alpha, nitrate, and malonaldehyde serum levels were determined. Hearts were taken from a further group of pretreated rabbits (n = 10) to assess myocardial COX-2 and HSP72 levels.-
dc.description.abstractGlutamine pretreatment resulted in a 39% reduction in infarct size (30.7 +/- 2.0% versus 50.4 +/- 2.1% controls; P < 0.01). Myocardial COX-2 levels were significantly elevated with pretreatment (P < 0.05) and were mirrored by higher serum 6-keto-PGF-1alpha levels prior to ischemia (69 +/- 13 versus 18 +/- 21 pg/mL in controls, P = 0.027). There was no significant difference in myocardial HSP72 or serum nitrate levels following pretreatment, or malonaldehyde levels during reperfusion.-
dc.description.abstractGlutamine pretreatment confers anti-infarct protection through up-regulation of COX-2, a key mediator of delayed preconditioning protection. Previous confirmation of its clinical safety profile at these doses suggests an acceptable strategy for inducing preconditioning for perioperative protection.-
dc.language.isoenen
dc.subject.mesh6-Ketoprostaglandin F1 alpha-
dc.subject.meshAnimals-
dc.subject.meshCyclooxygenase 2-
dc.subject.meshGlutamine-
dc.subject.meshHSP72 Heat-Shock Proteins-
dc.subject.meshHemodynamics-
dc.subject.meshInjections, Intravenous-
dc.subject.meshIschemic Preconditioning, Myocardial-
dc.subject.meshMale-
dc.subject.meshMalondialdehyde-
dc.subject.meshMyocardial Reperfusion Injury-
dc.subject.meshMyocardium-
dc.subject.meshNitrates-
dc.subject.meshRabbits-
dc.titleIntravenous glutamine enhances COX-2 activity giving cardioprotection.en
dc.typeArticleen
dc.contributor.departmentThe Royal College of Surgeons in Ireland, Department of Surgery, Beaumont Hospital, Dublin, Ireland.en
dc.identifier.journalThe Journal of surgical researchen
dc.description.provinceLeinster-

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