Role of potassium and calcium channels in sevoflurane-mediated vasodilation in the foeto-placental circulation.

Hdl Handle:
http://hdl.handle.net/10147/127673
Title:
Role of potassium and calcium channels in sevoflurane-mediated vasodilation in the foeto-placental circulation.
Authors:
Jarman, James; Maharaj, Chrisen H; Higgins, Brendan D; Farragher, Rachel F; Laffey, Christopher D; Flynn, Noel M; Laffey, John G
Affiliation:
Department of Anaesthesia, Clinical Sciences Institute, National University of Ireland, Galway Ireland. john.laffey@nuigalway.ie.
Citation:
Role of potassium and calcium channels in sevoflurane-mediated vasodilation in the foeto-placental circulation. 2009, 9:4 BMC Anesthesiol
Journal:
BMC anesthesiology
Issue Date:
2009
URI:
http://hdl.handle.net/10147/127673
DOI:
10.1186/1471-2253-9-4
PubMed ID:
19515255
Abstract:
ABSTRACT:; Sevoflurane has been demonstrated to vasodilate the foeto-placental vasculature. We aimed to determine the contribution of modulation of potassium and calcium channel function to the vasodilatory effect of sevoflurane in isolated human chorionic plate arterial rings.; Quadruplicate ex vivo human chorionic plate arterial rings were used in all studies. Series 1 and 2 examined the role of the K+ channel in sevoflurane-mediated vasodilation. Separate experiments examined whether tetraethylammonium, which blocks large conductance calcium activated K+ (KCa++) channels (Series 1A+B) or glibenclamide, which blocks the ATP sensitive K+ (KATP) channel (Series 2), modulated sevoflurane-mediated vasodilation. Series 3 - 5 examined the role of the Ca++ channel in sevoflurane induced vasodilation. Separate experiments examined whether verapamil, which blocks the sarcolemmal voltage-operated Ca++ channel (Series 3), SK&F 96365 an inhibitor of sarcolemmal voltage-independent Ca++ channels (Series 4A+B), or ryanodine an inhibitor of the sarcoplasmic reticulum Ca++ channel (Series 5A+B), modulated sevoflurane-mediated vasodilation.; Sevoflurane produced dose dependent vasodilatation of chorionic plate arterial rings in all studies. Prior blockade of the KCa++ and KATP channels augmented the vasodilator effects of sevoflurane. Furthermore, exposure of rings to sevoflurane in advance of TEA occluded the effects of TEA. Taken together, these findings suggest that sevoflurane blocks K+ channels. Blockade of the voltage-operated Ca++channels inhibited the vasodilator effects of sevoflurane. In contrast, blockade of the voltage-independent and sarcoplasmic reticulum Ca++channels did not alter sevoflurane vasodilation.; Sevoflurane appears to block chorionic arterial KCa++ and KATP channels. Sevoflurane also blocks voltage-operated calcium channels, and exerts a net vasodilatory effect in the in vitro foeto-placental circulation.
Item Type:
Article
Language:
en
ISSN:
1471-2253

Full metadata record

DC FieldValue Language
dc.contributor.authorJarman, Jamesen
dc.contributor.authorMaharaj, Chrisen Hen
dc.contributor.authorHiggins, Brendan Den
dc.contributor.authorFarragher, Rachel Fen
dc.contributor.authorLaffey, Christopher Den
dc.contributor.authorFlynn, Noel Men
dc.contributor.authorLaffey, John Gen
dc.date.accessioned2011-04-07T10:43:43Z-
dc.date.available2011-04-07T10:43:43Z-
dc.date.issued2009-
dc.identifier.citationRole of potassium and calcium channels in sevoflurane-mediated vasodilation in the foeto-placental circulation. 2009, 9:4 BMC Anesthesiolen
dc.identifier.issn1471-2253-
dc.identifier.pmid19515255-
dc.identifier.doi10.1186/1471-2253-9-4-
dc.identifier.urihttp://hdl.handle.net/10147/127673-
dc.description.abstractABSTRACT:-
dc.description.abstractSevoflurane has been demonstrated to vasodilate the foeto-placental vasculature. We aimed to determine the contribution of modulation of potassium and calcium channel function to the vasodilatory effect of sevoflurane in isolated human chorionic plate arterial rings.-
dc.description.abstractQuadruplicate ex vivo human chorionic plate arterial rings were used in all studies. Series 1 and 2 examined the role of the K+ channel in sevoflurane-mediated vasodilation. Separate experiments examined whether tetraethylammonium, which blocks large conductance calcium activated K+ (KCa++) channels (Series 1A+B) or glibenclamide, which blocks the ATP sensitive K+ (KATP) channel (Series 2), modulated sevoflurane-mediated vasodilation. Series 3 - 5 examined the role of the Ca++ channel in sevoflurane induced vasodilation. Separate experiments examined whether verapamil, which blocks the sarcolemmal voltage-operated Ca++ channel (Series 3), SK&F 96365 an inhibitor of sarcolemmal voltage-independent Ca++ channels (Series 4A+B), or ryanodine an inhibitor of the sarcoplasmic reticulum Ca++ channel (Series 5A+B), modulated sevoflurane-mediated vasodilation.-
dc.description.abstractSevoflurane produced dose dependent vasodilatation of chorionic plate arterial rings in all studies. Prior blockade of the KCa++ and KATP channels augmented the vasodilator effects of sevoflurane. Furthermore, exposure of rings to sevoflurane in advance of TEA occluded the effects of TEA. Taken together, these findings suggest that sevoflurane blocks K+ channels. Blockade of the voltage-operated Ca++channels inhibited the vasodilator effects of sevoflurane. In contrast, blockade of the voltage-independent and sarcoplasmic reticulum Ca++channels did not alter sevoflurane vasodilation.-
dc.description.abstractSevoflurane appears to block chorionic arterial KCa++ and KATP channels. Sevoflurane also blocks voltage-operated calcium channels, and exerts a net vasodilatory effect in the in vitro foeto-placental circulation.-
dc.language.isoenen
dc.titleRole of potassium and calcium channels in sevoflurane-mediated vasodilation in the foeto-placental circulation.en
dc.typeArticleen
dc.contributor.departmentDepartment of Anaesthesia, Clinical Sciences Institute, National University of Ireland, Galway Ireland. john.laffey@nuigalway.ie.en
dc.identifier.journalBMC anesthesiologyen
dc.description.provinceConnacht-

Related articles on PubMed

All Items in Lenus, The Irish Health Repository are protected by copyright, with all rights reserved, unless otherwise indicated.