Protein kinase D stabilizes aldosterone-induced ERK1/2 MAP kinase activation in M1 renal cortical collecting duct cells to promote cell proliferation.

Hdl Handle:
http://hdl.handle.net/10147/127246
Title:
Protein kinase D stabilizes aldosterone-induced ERK1/2 MAP kinase activation in M1 renal cortical collecting duct cells to promote cell proliferation.
Authors:
McEneaney, Victoria; Dooley, Ruth; Harvey, Brian J; Thomas, Warren
Affiliation:
Department of Molecular Medicine, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin 9, Ireland.
Citation:
Protein kinase D stabilizes aldosterone-induced ERK1/2 MAP kinase activation in M1 renal cortical collecting duct cells to promote cell proliferation. 2010, 118 (1-2):18-28 J. Steroid Biochem. Mol. Biol.
Journal:
The Journal of steroid biochemistry and molecular biology
Issue Date:
Jan-2010
URI:
http://hdl.handle.net/10147/127246
DOI:
10.1016/j.jsbmb.2009.09.014
PubMed ID:
19804826
Abstract:
Aldosterone elicits transcriptional responses in target tissues and also rapidly stimulates the activation of protein kinase signalling cascades independently of de novo protein synthesis. Here we investigated aldosterone-induced cell proliferation and extra-cellular regulated kinase 1 and 2 (ERK1/2) mitogen activated protein (MAP) kinase signalling in the M1 cortical collecting duct cell line (M1-CCD). Aldosterone promoted the proliferative growth of M1-CCD cells, an effect that was protein kinase D1 (PKD1), PKCdelta and ERK1/2-dependent. Aldosterone induced the rapid activation of ERK1/2 with peaks of activation at 2 and 10 to 30 min after hormone treatment followed by sustained activation lasting beyond 120 min. M1-CCD cells suppressed in PKD1 expression exhibited only the early, transient peaks in ERK1/2 activation without the sustained phase. Aldosterone stimulated the physical association of PKD1 with ERK1/2 within 2 min of treatment. The mineralocorticoid receptor (MR) antagonist RU28318 inhibited the early and late phases of aldosterone-induced ERK1/2 activation, and also aldosterone-induced proliferative cell growth. Aldosterone induced the sub-cellular redistribution of ERK1/2 to the nuclei at 2 min and to cytoplasmic sites, proximal to the nuclei after 30 min. This sub-cellular distribution of ERK1/2 was inhibited in cells suppressed in the expression of PKD1.
Item Type:
Article
Language:
en
MeSH:
Acetophenones; Active Transport, Cell Nucleus; Aldosterone; Aldosterone Antagonists; Animals; Benzopyrans; Cell Line; Cell Proliferation; Cytoplasm; Enzyme Activation; Epithelial Cells; Extracellular Signal-Regulated MAP Kinases; Flavonoids; Kidney Cortex; Kidney Tubules, Collecting; Mice; Mitogen-Activated Protein Kinase 1; Mitogen-Activated Protein Kinase 3; Models, Biological; Phosphorylation; Protein Binding; Protein Kinase C-delta; Protein Kinase Inhibitors; Receptor, Epidermal Growth Factor; Receptors, Mineralocorticoid; Signal Transduction; Spironolactone; TRPP Cation Channels; Tyrphostins
ISSN:
1879-1220

Full metadata record

DC FieldValue Language
dc.contributor.authorMcEneaney, Victoriaen
dc.contributor.authorDooley, Ruthen
dc.contributor.authorHarvey, Brian Jen
dc.contributor.authorThomas, Warrenen
dc.date.accessioned2011-04-05T15:23:40Z-
dc.date.available2011-04-05T15:23:40Z-
dc.date.issued2010-01-
dc.identifier.citationProtein kinase D stabilizes aldosterone-induced ERK1/2 MAP kinase activation in M1 renal cortical collecting duct cells to promote cell proliferation. 2010, 118 (1-2):18-28 J. Steroid Biochem. Mol. Biol.en
dc.identifier.issn1879-1220-
dc.identifier.pmid19804826-
dc.identifier.doi10.1016/j.jsbmb.2009.09.014-
dc.identifier.urihttp://hdl.handle.net/10147/127246-
dc.description.abstractAldosterone elicits transcriptional responses in target tissues and also rapidly stimulates the activation of protein kinase signalling cascades independently of de novo protein synthesis. Here we investigated aldosterone-induced cell proliferation and extra-cellular regulated kinase 1 and 2 (ERK1/2) mitogen activated protein (MAP) kinase signalling in the M1 cortical collecting duct cell line (M1-CCD). Aldosterone promoted the proliferative growth of M1-CCD cells, an effect that was protein kinase D1 (PKD1), PKCdelta and ERK1/2-dependent. Aldosterone induced the rapid activation of ERK1/2 with peaks of activation at 2 and 10 to 30 min after hormone treatment followed by sustained activation lasting beyond 120 min. M1-CCD cells suppressed in PKD1 expression exhibited only the early, transient peaks in ERK1/2 activation without the sustained phase. Aldosterone stimulated the physical association of PKD1 with ERK1/2 within 2 min of treatment. The mineralocorticoid receptor (MR) antagonist RU28318 inhibited the early and late phases of aldosterone-induced ERK1/2 activation, and also aldosterone-induced proliferative cell growth. Aldosterone induced the sub-cellular redistribution of ERK1/2 to the nuclei at 2 min and to cytoplasmic sites, proximal to the nuclei after 30 min. This sub-cellular distribution of ERK1/2 was inhibited in cells suppressed in the expression of PKD1.-
dc.language.isoenen
dc.subject.meshAcetophenones-
dc.subject.meshActive Transport, Cell Nucleus-
dc.subject.meshAldosterone-
dc.subject.meshAldosterone Antagonists-
dc.subject.meshAnimals-
dc.subject.meshBenzopyrans-
dc.subject.meshCell Line-
dc.subject.meshCell Proliferation-
dc.subject.meshCytoplasm-
dc.subject.meshEnzyme Activation-
dc.subject.meshEpithelial Cells-
dc.subject.meshExtracellular Signal-Regulated MAP Kinases-
dc.subject.meshFlavonoids-
dc.subject.meshKidney Cortex-
dc.subject.meshKidney Tubules, Collecting-
dc.subject.meshMice-
dc.subject.meshMitogen-Activated Protein Kinase 1-
dc.subject.meshMitogen-Activated Protein Kinase 3-
dc.subject.meshModels, Biological-
dc.subject.meshPhosphorylation-
dc.subject.meshProtein Binding-
dc.subject.meshProtein Kinase C-delta-
dc.subject.meshProtein Kinase Inhibitors-
dc.subject.meshReceptor, Epidermal Growth Factor-
dc.subject.meshReceptors, Mineralocorticoid-
dc.subject.meshSignal Transduction-
dc.subject.meshSpironolactone-
dc.subject.meshTRPP Cation Channels-
dc.subject.meshTyrphostins-
dc.titleProtein kinase D stabilizes aldosterone-induced ERK1/2 MAP kinase activation in M1 renal cortical collecting duct cells to promote cell proliferation.en
dc.typeArticleen
dc.contributor.departmentDepartment of Molecular Medicine, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin 9, Ireland.en
dc.identifier.journalThe Journal of steroid biochemistry and molecular biologyen
dc.description.provinceLeinster-
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