Taurine modulates neutrophil function but potentiates uropathogenic E. coli infection in the murine bladder.

Hdl Handle:
http://hdl.handle.net/10147/127198
Title:
Taurine modulates neutrophil function but potentiates uropathogenic E. coli infection in the murine bladder.
Authors:
Condron, Claire; Casey, Rowan G; Kehoe, Siobhan; Toomey, Deirdre; Creagh, Tom; Bouchier-Hayes, David J
Affiliation:
Department of Surgery, Royal College of Surgeons in Ireland, Beaumont Hospital, Dublin, Ireland.
Citation:
Taurine modulates neutrophil function but potentiates uropathogenic E. coli infection in the murine bladder. 2010, 38 (4):215-22 Urol. Res.
Journal:
Urological research
Issue Date:
Aug-2010
URI:
http://hdl.handle.net/10147/127198
DOI:
10.1007/s00240-009-0235-z
PubMed ID:
19940987
Abstract:
Eradication of a urinary tract infection (UTI) appears to be related to a number of innate host defence mechanisms and their interactions with invading bacteria. Recurrent UTIs (rUTIs) pose a difficult problem in that these bacteria use both host and bacterial factors to evade elimination. Neutrophil bactericidal function is depressed, both systemically and in urine, in patients with a history of recurrent UTI. Taurine is a semi-essential amino acid and is successful in preserving neutrophil bactericidal function in urine. Taurine may preserve neutrophil function at the urothelium and thus aid UTI resolution. Adult female (6 weeks old) C57Bl/6 mice were randomised into three groups: a saline gavage only control group, a saline gavage + E. coli group, and a taurine gavage + E. coli group [21 g/70 kg taurine in 0.9% normal saline (N/S) for 5 days]. Whilst taurine gavage pre-treatment resulted in increased serum neutrophils respiratory burst activity, at the urothelial-endothelial interface it caused higher colony forming units in the urine and a higher incidence of E. coli invasion in the bladder wall with no evidence of increased bladder wall neutrophils infiltration on MPO assay of histological assessment. Histologically there was also evidence of reduced bladder inflammation and urothelial cell apoptosis. In conclusion, taurine effectively increases neutrophils activity but given its anti-inflammatory properties, at the expense of decreased urothelial-endothelial activation thus preventing clearance of active E. coli infection in the bladder. Despite the negative results, this study demonstrates the importance of modulating interactions at the urothelial interface.
Item Type:
Article
Language:
en
MeSH:
Animals; Disease Models, Animal; Escherichia coli; Escherichia coli Infections; Female; Mice; Mice, Inbred C57BL; Neutrophil Infiltration; Neutrophils; Taurine; Urinary Bladder; Urinary Tract Infections; Urothelium
ISSN:
1434-0879

Full metadata record

DC FieldValue Language
dc.contributor.authorCondron, Claireen
dc.contributor.authorCasey, Rowan Gen
dc.contributor.authorKehoe, Siobhanen
dc.contributor.authorToomey, Deirdreen
dc.contributor.authorCreagh, Tomen
dc.contributor.authorBouchier-Hayes, David Jen
dc.date.accessioned2011-04-05T14:25:46Z-
dc.date.available2011-04-05T14:25:46Z-
dc.date.issued2010-08-
dc.identifier.citationTaurine modulates neutrophil function but potentiates uropathogenic E. coli infection in the murine bladder. 2010, 38 (4):215-22 Urol. Res.en
dc.identifier.issn1434-0879-
dc.identifier.pmid19940987-
dc.identifier.doi10.1007/s00240-009-0235-z-
dc.identifier.urihttp://hdl.handle.net/10147/127198-
dc.description.abstractEradication of a urinary tract infection (UTI) appears to be related to a number of innate host defence mechanisms and their interactions with invading bacteria. Recurrent UTIs (rUTIs) pose a difficult problem in that these bacteria use both host and bacterial factors to evade elimination. Neutrophil bactericidal function is depressed, both systemically and in urine, in patients with a history of recurrent UTI. Taurine is a semi-essential amino acid and is successful in preserving neutrophil bactericidal function in urine. Taurine may preserve neutrophil function at the urothelium and thus aid UTI resolution. Adult female (6 weeks old) C57Bl/6 mice were randomised into three groups: a saline gavage only control group, a saline gavage + E. coli group, and a taurine gavage + E. coli group [21 g/70 kg taurine in 0.9% normal saline (N/S) for 5 days]. Whilst taurine gavage pre-treatment resulted in increased serum neutrophils respiratory burst activity, at the urothelial-endothelial interface it caused higher colony forming units in the urine and a higher incidence of E. coli invasion in the bladder wall with no evidence of increased bladder wall neutrophils infiltration on MPO assay of histological assessment. Histologically there was also evidence of reduced bladder inflammation and urothelial cell apoptosis. In conclusion, taurine effectively increases neutrophils activity but given its anti-inflammatory properties, at the expense of decreased urothelial-endothelial activation thus preventing clearance of active E. coli infection in the bladder. Despite the negative results, this study demonstrates the importance of modulating interactions at the urothelial interface.-
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshDisease Models, Animal-
dc.subject.meshEscherichia coli-
dc.subject.meshEscherichia coli Infections-
dc.subject.meshFemale-
dc.subject.meshMice-
dc.subject.meshMice, Inbred C57BL-
dc.subject.meshNeutrophil Infiltration-
dc.subject.meshNeutrophils-
dc.subject.meshTaurine-
dc.subject.meshUrinary Bladder-
dc.subject.meshUrinary Tract Infections-
dc.subject.meshUrothelium-
dc.titleTaurine modulates neutrophil function but potentiates uropathogenic E. coli infection in the murine bladder.en
dc.typeArticleen
dc.contributor.departmentDepartment of Surgery, Royal College of Surgeons in Ireland, Beaumont Hospital, Dublin, Ireland.en
dc.identifier.journalUrological researchen
dc.description.provinceLeinster-

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