The Beckman DxI 800 prolactin assay demonstrates superior specificity for monomeric prolactin.

Hdl Handle:
http://hdl.handle.net/10147/127197
Title:
The Beckman DxI 800 prolactin assay demonstrates superior specificity for monomeric prolactin.
Authors:
Byrne, Brendan; O'Shea, Paula; Barrett, Patricia; Tormey, William
Affiliation:
Department of Chemical Pathology, Beaumont Hospital, Dublin, Ireland. brendanbyrne@beaumont.ie
Citation:
The Beckman DxI 800 prolactin assay demonstrates superior specificity for monomeric prolactin. 2010, 48 (2):205-8 Clin. Chem. Lab. Med.
Journal:
Clinical chemistry and laboratory medicine : CCLM / FESCC
Issue Date:
Feb-2010
URI:
http://hdl.handle.net/10147/127197
DOI:
10.1515/CCLM.2010.038
PubMed ID:
19943807
Abstract:
Commercially available prolactin immunoassays detect macroprolactin to variable degrees. Best practice requires laboratories to assess the cross-reactivity of their prolactin assay with macroprolactin, and where appropriate, introduce a screen for the presence of macroprolactin. Our policy has been to reanalyse hyperprolactinaemic samples following polyethylene glycol (PEG) precipitation and to report the resultant value as the monomeric prolactin content of the sample. The goal of this study was to determine the need to continue PEG precipitation when prolactin measurements with the Wallac AutoDELFIA were replaced by the Beckman DxI 800.; A total of 317 apparently hyperprolactinaemic samples were analysed for prolactin using the Beckman DxI 800 and results compared with those determined with the PEG screening technique on the Wallac AutoDELFIA. Any samples demonstrating a discordance of >25% were re-analysed using gel filtration chromatography (GFC) for a definitive result.; The results indicate the Beckman DxI overestimates the prolactin concentration in 1%-2% of hyperprolactinaemic samples. PEG precipitation prior to analysis with the Wallac AutoDELFIA resulted in a 1% false negative diagnosis of hyperprolactinaemia.; Prolactin results can be reported directly from the DxI. When results are discordant with the clinical presentation, prolactin should be measured using GFC.
Item Type:
Article
Language:
en
MeSH:
Chemical Precipitation; Chromatography, Gel; Clinical Laboratory Techniques; False Positive Reactions; Female; Humans; Hyperprolactinemia; Immunoassay; Male; Polyethylene Glycols; Prolactin; Reagent Kits, Diagnostic; Reproducibility of Results; Sensitivity and Specificity
ISSN:
1434-6621

Full metadata record

DC FieldValue Language
dc.contributor.authorByrne, Brendanen
dc.contributor.authorO'Shea, Paulaen
dc.contributor.authorBarrett, Patriciaen
dc.contributor.authorTormey, Williamen
dc.date.accessioned2011-04-05T14:25:01Z-
dc.date.available2011-04-05T14:25:01Z-
dc.date.issued2010-02-
dc.identifier.citationThe Beckman DxI 800 prolactin assay demonstrates superior specificity for monomeric prolactin. 2010, 48 (2):205-8 Clin. Chem. Lab. Med.en
dc.identifier.issn1434-6621-
dc.identifier.pmid19943807-
dc.identifier.doi10.1515/CCLM.2010.038-
dc.identifier.urihttp://hdl.handle.net/10147/127197-
dc.description.abstractCommercially available prolactin immunoassays detect macroprolactin to variable degrees. Best practice requires laboratories to assess the cross-reactivity of their prolactin assay with macroprolactin, and where appropriate, introduce a screen for the presence of macroprolactin. Our policy has been to reanalyse hyperprolactinaemic samples following polyethylene glycol (PEG) precipitation and to report the resultant value as the monomeric prolactin content of the sample. The goal of this study was to determine the need to continue PEG precipitation when prolactin measurements with the Wallac AutoDELFIA were replaced by the Beckman DxI 800.-
dc.description.abstractA total of 317 apparently hyperprolactinaemic samples were analysed for prolactin using the Beckman DxI 800 and results compared with those determined with the PEG screening technique on the Wallac AutoDELFIA. Any samples demonstrating a discordance of >25% were re-analysed using gel filtration chromatography (GFC) for a definitive result.-
dc.description.abstractThe results indicate the Beckman DxI overestimates the prolactin concentration in 1%-2% of hyperprolactinaemic samples. PEG precipitation prior to analysis with the Wallac AutoDELFIA resulted in a 1% false negative diagnosis of hyperprolactinaemia.-
dc.description.abstractProlactin results can be reported directly from the DxI. When results are discordant with the clinical presentation, prolactin should be measured using GFC.-
dc.language.isoenen
dc.subject.meshChemical Precipitation-
dc.subject.meshChromatography, Gel-
dc.subject.meshClinical Laboratory Techniques-
dc.subject.meshFalse Positive Reactions-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshHyperprolactinemia-
dc.subject.meshImmunoassay-
dc.subject.meshMale-
dc.subject.meshPolyethylene Glycols-
dc.subject.meshProlactin-
dc.subject.meshReagent Kits, Diagnostic-
dc.subject.meshReproducibility of Results-
dc.subject.meshSensitivity and Specificity-
dc.titleThe Beckman DxI 800 prolactin assay demonstrates superior specificity for monomeric prolactin.en
dc.typeArticleen
dc.contributor.departmentDepartment of Chemical Pathology, Beaumont Hospital, Dublin, Ireland. brendanbyrne@beaumont.ieen
dc.identifier.journalClinical chemistry and laboratory medicine : CCLM / FESCCen
dc.description.provinceLeinster-

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