Contrast-induced nephrotoxicity: possible synergistic effect of stress hyperglycemia.

Hdl Handle:
http://hdl.handle.net/10147/126506
Title:
Contrast-induced nephrotoxicity: possible synergistic effect of stress hyperglycemia.
Authors:
O'Donnell, David H; Moloney, Michael A; Bouchier-Hayes, David J; Lee, Michael J
Affiliation:
Department of Radiology and Vascular Surgery, Beaumont Hospital, The Royal College of Surgeons in Ireland, Dublin, Ireland. davidhodonnell@hotmail.com <davidhodonnell@hotmail.com>
Citation:
Contrast-induced nephrotoxicity: possible synergistic effect of stress hyperglycemia. 2010, 195 (1):W45-9 AJR Am J Roentgenol
Journal:
AJR. American journal of roentgenology
Issue Date:
Jul-2010
URI:
http://hdl.handle.net/10147/126506
DOI:
10.2214/AJR.09.2847
PubMed ID:
20566779
Abstract:
Oxidative stress on the renal tubules has been implicated as a mechanism of injury in both stress hyperglycemia and contrast-induced nephrotoxicity. The purpose of this study was to determine whether the combination of these effects has a synergistic effect on accentuating renal tubular apoptosis and therefore increasing the risk of contrast-induced nephrotoxicity.; Dog kidney cells were incubated with 5- and 30-mmol/L glucose solutions and no glucose and then exposed for 2 hours to three types of contrast media-high osmolar (370 mg I/mL), low osmolar (300 mg I/mL), and isoosmolar (320 mg I/mL)-and a mannitol control solution. In an identical experiment, each group of cells was pretreated with an antioxidant-N-acetylcysteine or taurine-to evaluate the protective effect, if any. Apoptosis was assessed with fluorescence-activated cell sorter flow cytometry.; The high-osmolar contrast medium was associated with significantly elevated levels of apoptosis compared with the mannitol control (percentage apoptosis, 27.98 +/- 1.08 vs 6.19 +/- 0.771; p < 0.001). This effect was less pronounced after incubation with the low-osmolar agent but was still significant (percentage apoptosis, 20.19 +/- 0.3665 vs 6.19 +/- 0.771; p < 0.001). The isosmolar agent did not have a significant effect. Both the high- and low-osmolar contrast media coupled with hyperglycemia (30-mmol/L glucose) were associated with a significantly increased level of apoptosis. In all contrast medium groups, taurine had a greater protective effect on attenuation of cell apoptosis than did N-acetylcysteine.; The combination of contrast medium and an elevated glucose level has a synergistic effect on apoptosis. Taurine may be a more effective prophylactic antioxidant than the currently advocated antioxidant, N-acetylcysteine.
Item Type:
Article
Language:
en
MeSH:
Acetylcysteine; Analysis of Variance; Animals; Apoptosis; Contrast Media; Dogs; Hyperglycemia; Kidney Diseases; Kidney Tubules; Necrosis; Osmolar Concentration; Oxidative Stress; Taurine
ISSN:
1546-3141

Full metadata record

DC FieldValue Language
dc.contributor.authorO'Donnell, David Hen
dc.contributor.authorMoloney, Michael Aen
dc.contributor.authorBouchier-Hayes, David Jen
dc.contributor.authorLee, Michael Jen
dc.date.accessioned2011-03-31T09:25:43Z-
dc.date.available2011-03-31T09:25:43Z-
dc.date.issued2010-07-
dc.identifier.citationContrast-induced nephrotoxicity: possible synergistic effect of stress hyperglycemia. 2010, 195 (1):W45-9 AJR Am J Roentgenolen
dc.identifier.issn1546-3141-
dc.identifier.pmid20566779-
dc.identifier.doi10.2214/AJR.09.2847-
dc.identifier.urihttp://hdl.handle.net/10147/126506-
dc.description.abstractOxidative stress on the renal tubules has been implicated as a mechanism of injury in both stress hyperglycemia and contrast-induced nephrotoxicity. The purpose of this study was to determine whether the combination of these effects has a synergistic effect on accentuating renal tubular apoptosis and therefore increasing the risk of contrast-induced nephrotoxicity.-
dc.description.abstractDog kidney cells were incubated with 5- and 30-mmol/L glucose solutions and no glucose and then exposed for 2 hours to three types of contrast media-high osmolar (370 mg I/mL), low osmolar (300 mg I/mL), and isoosmolar (320 mg I/mL)-and a mannitol control solution. In an identical experiment, each group of cells was pretreated with an antioxidant-N-acetylcysteine or taurine-to evaluate the protective effect, if any. Apoptosis was assessed with fluorescence-activated cell sorter flow cytometry.-
dc.description.abstractThe high-osmolar contrast medium was associated with significantly elevated levels of apoptosis compared with the mannitol control (percentage apoptosis, 27.98 +/- 1.08 vs 6.19 +/- 0.771; p < 0.001). This effect was less pronounced after incubation with the low-osmolar agent but was still significant (percentage apoptosis, 20.19 +/- 0.3665 vs 6.19 +/- 0.771; p < 0.001). The isosmolar agent did not have a significant effect. Both the high- and low-osmolar contrast media coupled with hyperglycemia (30-mmol/L glucose) were associated with a significantly increased level of apoptosis. In all contrast medium groups, taurine had a greater protective effect on attenuation of cell apoptosis than did N-acetylcysteine.-
dc.description.abstractThe combination of contrast medium and an elevated glucose level has a synergistic effect on apoptosis. Taurine may be a more effective prophylactic antioxidant than the currently advocated antioxidant, N-acetylcysteine.-
dc.language.isoenen
dc.subject.meshAcetylcysteine-
dc.subject.meshAnalysis of Variance-
dc.subject.meshAnimals-
dc.subject.meshApoptosis-
dc.subject.meshContrast Media-
dc.subject.meshDogs-
dc.subject.meshHyperglycemia-
dc.subject.meshKidney Diseases-
dc.subject.meshKidney Tubules-
dc.subject.meshNecrosis-
dc.subject.meshOsmolar Concentration-
dc.subject.meshOxidative Stress-
dc.subject.meshTaurine-
dc.titleContrast-induced nephrotoxicity: possible synergistic effect of stress hyperglycemia.en
dc.typeArticleen
dc.contributor.departmentDepartment of Radiology and Vascular Surgery, Beaumont Hospital, The Royal College of Surgeons in Ireland, Dublin, Ireland. davidhodonnell@hotmail.com <davidhodonnell@hotmail.com>en
dc.identifier.journalAJR. American journal of roentgenologyen
dc.description.provinceLeinster-

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