Evaluation of Zinc-alpha-2-Glycoprotein and Proteasome Subunit beta-Type 6 Expression in Prostate Cancer Using Tissue Microarray Technology.

Hdl Handle:
http://hdl.handle.net/10147/126451
Title:
Evaluation of Zinc-alpha-2-Glycoprotein and Proteasome Subunit beta-Type 6 Expression in Prostate Cancer Using Tissue Microarray Technology.
Authors:
Oʼhurley, Gillian; Oʼgrady, Anthony; Smyth, Paul; Byrne, Jennifer; Oʼleary, John J; Sheils, Orla; Watson, R William G; Kay, Elaine W
Affiliation:
Department of Pathology, RCSI ERC Beaumont Hospital daggerDepartment of Histopathology, Trinity College Dublin double daggerUCD School of Medicine and Medical Science, UCD Conway Institute of Biomolecular and Biomedical Research, Universtity College Dublin, Ireland.
Citation:
Evaluation of Zinc-alpha-2-Glycoprotein and Proteasome Subunit beta-Type 6 Expression in Prostate Cancer Using Tissue Microarray Technology. 2010: Appl. Immunohistochem. Mol. Morphol.
Journal:
Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry
Issue Date:
23-Jul-2010
URI:
http://hdl.handle.net/10147/126451
DOI:
10.1097/PAI.0b013e3181e29998
PubMed ID:
20661134
Abstract:
Prostate cancer (CaP) is a significant cause of illness and death in males. Current detection strategies do not reliably detect the disease at an early stage and cannot distinguish aggressive versus nonaggressive CaP leading to potential overtreatment of the disease and associated morbidity. Zinc-alpha-2-glycoprotein (ZAG) and proteasome subunit beta-Type 6 (PSMB-6) were found to be up-regulated in the serum of CaP patients with higher grade tumors after 2-dimensional difference gel electrophoresis analysis. The aim of this study was to investigate if ZAG and PSMB-6 were also overexpressed in prostatic tumor tissue of CaP patients. Immunohistochemical analysis was performed on CaP tissue microarrays with samples from 199 patients. Confirmatory gene expression profiling for ZAG and PSMB-6 were performed on 4 cases using Laser Capture Microdissection and TaqMan real-time polymerase chain reaction. ZAG expression in CaP epithelial cells was inversely associated with Gleason grade (benign prostatic hyperplasia>G3>G4/G5). PSMB-6 was not expressed in either tumor or benign epithelium. However, strong PSMB-6 expression was noted in stromal and inflammatory cells. Our results indicate ZAG as a possible predictive marker of Gleason grade. The inverse association between grade and tissue expression with a rising serum protein level is similar to that seen with prostate-specific antigen. In addition, the results for both ZAG and PSMB-6 highlight the challenges in trying to associate the protein levels in serum with tissue expression.
Item Type:
Article In Press
Language:
null
ISSN:
1533-4058

Full metadata record

DC FieldValue Language
dc.contributor.authorOʼhurley, Gillianen
dc.contributor.authorOʼgrady, Anthonyen
dc.contributor.authorSmyth, Paulen
dc.contributor.authorByrne, Jenniferen
dc.contributor.authorOʼleary, John Jen
dc.contributor.authorSheils, Orlaen
dc.contributor.authorWatson, R William Gen
dc.contributor.authorKay, Elaine Wen
dc.date.accessioned2011-03-31T08:36:13Z-
dc.date.available2011-03-31T08:36:13Z-
dc.date.issued2010-07-23-
dc.identifier.citationEvaluation of Zinc-alpha-2-Glycoprotein and Proteasome Subunit beta-Type 6 Expression in Prostate Cancer Using Tissue Microarray Technology. 2010: Appl. Immunohistochem. Mol. Morphol.en
dc.identifier.issn1533-4058-
dc.identifier.pmid20661134-
dc.identifier.doi10.1097/PAI.0b013e3181e29998-
dc.identifier.urihttp://hdl.handle.net/10147/126451-
dc.description.abstractProstate cancer (CaP) is a significant cause of illness and death in males. Current detection strategies do not reliably detect the disease at an early stage and cannot distinguish aggressive versus nonaggressive CaP leading to potential overtreatment of the disease and associated morbidity. Zinc-alpha-2-glycoprotein (ZAG) and proteasome subunit beta-Type 6 (PSMB-6) were found to be up-regulated in the serum of CaP patients with higher grade tumors after 2-dimensional difference gel electrophoresis analysis. The aim of this study was to investigate if ZAG and PSMB-6 were also overexpressed in prostatic tumor tissue of CaP patients. Immunohistochemical analysis was performed on CaP tissue microarrays with samples from 199 patients. Confirmatory gene expression profiling for ZAG and PSMB-6 were performed on 4 cases using Laser Capture Microdissection and TaqMan real-time polymerase chain reaction. ZAG expression in CaP epithelial cells was inversely associated with Gleason grade (benign prostatic hyperplasia>G3>G4/G5). PSMB-6 was not expressed in either tumor or benign epithelium. However, strong PSMB-6 expression was noted in stromal and inflammatory cells. Our results indicate ZAG as a possible predictive marker of Gleason grade. The inverse association between grade and tissue expression with a rising serum protein level is similar to that seen with prostate-specific antigen. In addition, the results for both ZAG and PSMB-6 highlight the challenges in trying to associate the protein levels in serum with tissue expression.-
dc.languageENG-
dc.language.isonullen
dc.titleEvaluation of Zinc-alpha-2-Glycoprotein and Proteasome Subunit beta-Type 6 Expression in Prostate Cancer Using Tissue Microarray Technology.en
dc.typeArticle In Pressen
dc.contributor.departmentDepartment of Pathology, RCSI ERC Beaumont Hospital daggerDepartment of Histopathology, Trinity College Dublin double daggerUCD School of Medicine and Medical Science, UCD Conway Institute of Biomolecular and Biomedical Research, Universtity College Dublin, Ireland.en
dc.identifier.journalApplied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistryen
dc.description.provinceLeinster-

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