Measurement of the unfolded protein response (UPR) in monocytes.

Hdl Handle:
http://hdl.handle.net/10147/126092
Title:
Measurement of the unfolded protein response (UPR) in monocytes.
Authors:
Carroll, Tomás P; Greene, Catherine M; McElvaney, Noel G
Affiliation:
Department of Medicine, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin, Ireland.
Citation:
Measurement of the unfolded protein response (UPR) in monocytes. 2011, 489:83-95 Meth. Enzymol.
Journal:
Methods in enzymology
Issue Date:
2011
URI:
http://hdl.handle.net/10147/126092
DOI:
10.1016/B978-0-12-385116-1.00005-4
PubMed ID:
21266225
Abstract:
In mammalian cells, the primary function of the endoplasmic reticulum (ER) is to synthesize and assemble membrane and secreted proteins. As the main site of protein folding and posttranslational modification in the cell, the ER operates a highly conserved quality control system to ensure only correctly assembled proteins exit the ER and misfolded and unfolded proteins are retained for disposal. Any disruption in the equilibrium of the ER engages a multifaceted intracellular signaling pathway termed the unfolded protein response (UPR) to restore normal conditions in the cell. A variety of pathological conditions can induce activation of the UPR, including neurodegenerative disorders such as Parkinson's disease, metabolic disorders such as atherosclerosis, and conformational disorders such as cystic fibrosis. Conformational disorders are characterized by mutations that modify the final structure of a protein and any cells that express abnormal protein risk functional impairment. The monocyte is an important and long-lived immune cell and acts as a key immunological orchestrator, dictating the intensity and duration of the host immune response. Monocytes expressing misfolded or unfolded protein may exhibit UPR activation and this can compromise the host immune system. Here, we describe in detail methods and protocols for the examination of UPR activation in peripheral blood monocytes. This guide should provide new investigators to the field with a broad understanding of the tools required to investigate the UPR in the monocyte.
Item Type:
Article
Language:
en
ISSN:
1557-7988

Full metadata record

DC FieldValue Language
dc.contributor.authorCarroll, Tomás Pen
dc.contributor.authorGreene, Catherine Men
dc.contributor.authorMcElvaney, Noel Gen
dc.date.accessioned2011-03-29T13:45:59Z-
dc.date.available2011-03-29T13:45:59Z-
dc.date.issued2011-
dc.identifier.citationMeasurement of the unfolded protein response (UPR) in monocytes. 2011, 489:83-95 Meth. Enzymol.en
dc.identifier.issn1557-7988-
dc.identifier.pmid21266225-
dc.identifier.doi10.1016/B978-0-12-385116-1.00005-4-
dc.identifier.urihttp://hdl.handle.net/10147/126092-
dc.description.abstractIn mammalian cells, the primary function of the endoplasmic reticulum (ER) is to synthesize and assemble membrane and secreted proteins. As the main site of protein folding and posttranslational modification in the cell, the ER operates a highly conserved quality control system to ensure only correctly assembled proteins exit the ER and misfolded and unfolded proteins are retained for disposal. Any disruption in the equilibrium of the ER engages a multifaceted intracellular signaling pathway termed the unfolded protein response (UPR) to restore normal conditions in the cell. A variety of pathological conditions can induce activation of the UPR, including neurodegenerative disorders such as Parkinson's disease, metabolic disorders such as atherosclerosis, and conformational disorders such as cystic fibrosis. Conformational disorders are characterized by mutations that modify the final structure of a protein and any cells that express abnormal protein risk functional impairment. The monocyte is an important and long-lived immune cell and acts as a key immunological orchestrator, dictating the intensity and duration of the host immune response. Monocytes expressing misfolded or unfolded protein may exhibit UPR activation and this can compromise the host immune system. Here, we describe in detail methods and protocols for the examination of UPR activation in peripheral blood monocytes. This guide should provide new investigators to the field with a broad understanding of the tools required to investigate the UPR in the monocyte.-
dc.language.isoenen
dc.titleMeasurement of the unfolded protein response (UPR) in monocytes.en
dc.typeArticleen
dc.contributor.departmentDepartment of Medicine, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin, Ireland.en
dc.identifier.journalMethods in enzymologyen
dc.description.provinceLeinster-

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