Characterization of a Novel Arginine Catabolic Mobile Element (ACME) and Staphylococcal Chromosomal Cassette mec Composite Island with Significant Homology to Staphylococcus epidermidis ACME type II in Methicillin-Resistant Staphylococcus aureus Genotype ST22-MRSA-IV.

Hdl Handle:
http://hdl.handle.net/10147/126075
Title:
Characterization of a Novel Arginine Catabolic Mobile Element (ACME) and Staphylococcal Chromosomal Cassette mec Composite Island with Significant Homology to Staphylococcus epidermidis ACME type II in Methicillin-Resistant Staphylococcus aureus Genotype ST22-MRSA-IV.
Authors:
Shore, Anna C; Rossney, Angela S; Brennan, Orla M; Kinnevey, Peter M; Humphreys, Hilary; Sullivan, Derek J; Goering, Richard V; Ehricht, Ralf; Monecke, Stefan; Coleman, David C
Affiliation:
Microbiology Research Unit, Dublin Dental University Hospital, University of Dublin, Trinity College Dublin, Ireland; National MRSA Reference Laboratory, Dublin, Ireland; Department of Clinical Microbiology, The Royal College of Surgeons in Ireland, Dublin, Ireland; Department of Microbiology, Beaumont Hospital, Dublin, Ireland; Creighton University, Omaha, Nebraska, USA; Alere Technologies GmbH, Jena, Germany; Institute for Medical Microbiology and Hygiene, Faculty of Medicine "Carl Gustav Carus", Technical University of Dresden, Germany.
Citation:
Characterization of a Novel Arginine Catabolic Mobile Element (ACME) and Staphylococcal Chromosomal Cassette mec Composite Island with Significant Homology to Staphylococcus epidermidis ACME type II in Methicillin-Resistant Staphylococcus aureus Genotype ST22-MRSA-IV. 2011:notAntimicrob Agents Chemother
Journal:
Antimicrobial agents and chemotherapy
Issue Date:
22-Feb-2011
URI:
http://hdl.handle.net/10147/126075
DOI:
10.1128/AAC.01756-10
PubMed ID:
21343442
Additional Links:
http://aac.asm.org/cgi/reprint/AAC.01756-10v1?view=long&pmid=21343442
Abstract:
The arginine catabolic mobile element (ACME) is prevalent among ST8-MRSA-IVa (USA300) isolates and evidence suggests that ACME enhances the ability of ST8-MRSA-IVa to grow and survive on its host. ACME has been identified in a small number of isolates belonging to other MRSA clones but is widespread among coagulase-negative staphylococci (CoNS). This study reports the first description of ACME in two distinct strains of the pandemic ST22-MRSA-IV clone. A total of 238 MRSA isolates recovered in Ireland between 1971 and 2008 were investigated for ACME using a DNA microarray. Twenty-three isolates (9.7%) were ACME-positive, all were either MRSA genotype ST8-MRSA-IVa (7/23, 30%) or ST22-MRSA-IV (16/23, 70%). Whole-genome sequencing and comprehensive molecular characterization revealed the presence of a novel 46-kb ACME and SCCmec composite island (ACME/SCCmec-CI) in ST22-MRSA-IVh isolates (n = 15). This ACME/SCCmec-CI consists of a 12-kb DNA region previously identified in ACME type II in S. epidermidis ATCC 12228, a truncated copy of the J1 region of SCCmec I and a complete SCCmec IVh element. The composite island has a novel genetic organization with ACME located within orfX and SCCmec located downstream of ACME. One pvl-positive ST22-MRSA-IVa isolate carried ACME located downstream of SCCmec IVa as previously described in ST8-MRSA-IVa. These results suggest that ACME has been acquired by ST22-MRSA-IV on two independent occasions. At least one of these instances may have involved horizontal transfer and recombination events between MRSA and CoNS. The presence of ACME may enhance dissemination of ST22-MRSA-IV, an already successful MRSA clone.
Item Type:
Article in Press
Language:
null
ISSN:
1098-6596

Full metadata record

DC FieldValue Language
dc.contributor.authorShore, Anna Cen
dc.contributor.authorRossney, Angela Sen
dc.contributor.authorBrennan, Orla Men
dc.contributor.authorKinnevey, Peter Men
dc.contributor.authorHumphreys, Hilaryen
dc.contributor.authorSullivan, Derek Jen
dc.contributor.authorGoering, Richard Ven
dc.contributor.authorEhricht, Ralfen
dc.contributor.authorMonecke, Stefanen
dc.contributor.authorColeman, David Cen
dc.date.accessioned2011-03-29T13:47:33Z-
dc.date.available2011-03-29T13:47:33Z-
dc.date.issued2011-02-22-
dc.identifier.citationCharacterization of a Novel Arginine Catabolic Mobile Element (ACME) and Staphylococcal Chromosomal Cassette mec Composite Island with Significant Homology to Staphylococcus epidermidis ACME type II in Methicillin-Resistant Staphylococcus aureus Genotype ST22-MRSA-IV. 2011:notAntimicrob Agents Chemotheren
dc.identifier.issn1098-6596-
dc.identifier.pmid21343442-
dc.identifier.doi10.1128/AAC.01756-10-
dc.identifier.urihttp://hdl.handle.net/10147/126075-
dc.description.abstractThe arginine catabolic mobile element (ACME) is prevalent among ST8-MRSA-IVa (USA300) isolates and evidence suggests that ACME enhances the ability of ST8-MRSA-IVa to grow and survive on its host. ACME has been identified in a small number of isolates belonging to other MRSA clones but is widespread among coagulase-negative staphylococci (CoNS). This study reports the first description of ACME in two distinct strains of the pandemic ST22-MRSA-IV clone. A total of 238 MRSA isolates recovered in Ireland between 1971 and 2008 were investigated for ACME using a DNA microarray. Twenty-three isolates (9.7%) were ACME-positive, all were either MRSA genotype ST8-MRSA-IVa (7/23, 30%) or ST22-MRSA-IV (16/23, 70%). Whole-genome sequencing and comprehensive molecular characterization revealed the presence of a novel 46-kb ACME and SCCmec composite island (ACME/SCCmec-CI) in ST22-MRSA-IVh isolates (n = 15). This ACME/SCCmec-CI consists of a 12-kb DNA region previously identified in ACME type II in S. epidermidis ATCC 12228, a truncated copy of the J1 region of SCCmec I and a complete SCCmec IVh element. The composite island has a novel genetic organization with ACME located within orfX and SCCmec located downstream of ACME. One pvl-positive ST22-MRSA-IVa isolate carried ACME located downstream of SCCmec IVa as previously described in ST8-MRSA-IVa. These results suggest that ACME has been acquired by ST22-MRSA-IV on two independent occasions. At least one of these instances may have involved horizontal transfer and recombination events between MRSA and CoNS. The presence of ACME may enhance dissemination of ST22-MRSA-IV, an already successful MRSA clone.-
dc.languageENG-
dc.language.isonullen
dc.relation.urlhttp://aac.asm.org/cgi/reprint/AAC.01756-10v1?view=long&pmid=21343442en
dc.titleCharacterization of a Novel Arginine Catabolic Mobile Element (ACME) and Staphylococcal Chromosomal Cassette mec Composite Island with Significant Homology to Staphylococcus epidermidis ACME type II in Methicillin-Resistant Staphylococcus aureus Genotype ST22-MRSA-IV.en
dc.typeArticle in Press-
dc.contributor.departmentMicrobiology Research Unit, Dublin Dental University Hospital, University of Dublin, Trinity College Dublin, Ireland; National MRSA Reference Laboratory, Dublin, Ireland; Department of Clinical Microbiology, The Royal College of Surgeons in Ireland, Dublin, Ireland; Department of Microbiology, Beaumont Hospital, Dublin, Ireland; Creighton University, Omaha, Nebraska, USA; Alere Technologies GmbH, Jena, Germany; Institute for Medical Microbiology and Hygiene, Faculty of Medicine "Carl Gustav Carus", Technical University of Dresden, Germany.en
dc.identifier.journalAntimicrobial agents and chemotherapyen
dc.description.provinceLeinster-
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