Dysregulated miR-183 inhibits migration in breast cancer cells.

Hdl Handle:
http://hdl.handle.net/10147/125852
Title:
Dysregulated miR-183 inhibits migration in breast cancer cells.
Authors:
Lowery, Aoife J; Miller, Nicola; Dwyer, Roisin M; Kerin, Michael J
Affiliation:
Department of Surgery, National University of Ireland, Galway, Ireland.
Citation:
Dysregulated miR-183 inhibits migration in breast cancer cells. 2010, 10:502 BMC Cancer
Journal:
BMC cancer
Issue Date:
2010
URI:
http://hdl.handle.net/10147/125852
DOI:
10.1186/1471-2407-10-502
PubMed ID:
20858276
Abstract:
The involvement of miRNAs in the regulation of fundamental cellular functions has placed them at the fore of ongoing investigations into the processes underlying carcinogenesis. MiRNA expression patterns have been shown to be dysregulated in numerous human malignancies, including breast cancer, suggesting their probable involvement as novel classes of oncogenes or tumour suppressor genes. The identification of differentially expressed miRNAs and elucidation of their functional roles may provide insight into the complex and diverse molecular mechanisms of tumorigenesis. MiR-183 is located on chromosome 7q32 and is part of a miRNA family which are dysregulated in numerous cancers. The aims of this study were to further examine the expression and functional role of miR-183 in breast cancer.; MiR-183 expression was quantitated in primary breast tumours, tumour associated normal tissue and breast cancer cell lines using RQ-PCR. Gain of function analysis was performed in breast cancer cells using pre-miR-183 and the effect of miR-183 overexpression on cell viability, proliferation, apoptosis and migration was examined. Customized Taqman Low Density Arrays (TLDA) were used to identify dysregulated genes in breast cancer cells transfected with pre-miR-183.; We demonstrate that miR-183 is dysregulated in breast cancer and expression correlates with estrogen receptor and HER2/neu receptor expression. Induced overexpression of miR-183 inhibited migration of breast cancer cells. This finding was substantiated by RQ-PCR of mRNA from cells overexpressing miR-183 which showed dysregulation of several migration and invasion related genes. Specifically, the VIL2-coding protein Ezrin was confirmed as a target of miR-183 and downregulation of this protein was confirmed with immunocytochemistry.; These findings indicate that miR-183 targets VIL2 and may play a central role in the regulation of migration and metastasis in breast cancer. Consequently, this miRNA may present an attractive target for therapeutic intervention.
Item Type:
Article
Language:
en
MeSH:
Apoptosis; Breast Neoplasms; Cell Line, Tumor; Cell Movement; Cytoskeletal Proteins; Down-Regulation; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; MicroRNAs; Neoplasm Invasiveness; Neoplasm Metastasis; RNA, Messenger; Reverse Transcriptase Polymerase Chain Reaction
ISSN:
1471-2407

Full metadata record

DC FieldValue Language
dc.contributor.authorLowery, Aoife Jen
dc.contributor.authorMiller, Nicolaen
dc.contributor.authorDwyer, Roisin Men
dc.contributor.authorKerin, Michael Jen
dc.date.accessioned2011-03-28T14:33:19Z-
dc.date.available2011-03-28T14:33:19Z-
dc.date.issued2010-
dc.identifier.citationDysregulated miR-183 inhibits migration in breast cancer cells. 2010, 10:502 BMC Canceren
dc.identifier.issn1471-2407-
dc.identifier.pmid20858276-
dc.identifier.doi10.1186/1471-2407-10-502-
dc.identifier.urihttp://hdl.handle.net/10147/125852-
dc.description.abstractThe involvement of miRNAs in the regulation of fundamental cellular functions has placed them at the fore of ongoing investigations into the processes underlying carcinogenesis. MiRNA expression patterns have been shown to be dysregulated in numerous human malignancies, including breast cancer, suggesting their probable involvement as novel classes of oncogenes or tumour suppressor genes. The identification of differentially expressed miRNAs and elucidation of their functional roles may provide insight into the complex and diverse molecular mechanisms of tumorigenesis. MiR-183 is located on chromosome 7q32 and is part of a miRNA family which are dysregulated in numerous cancers. The aims of this study were to further examine the expression and functional role of miR-183 in breast cancer.-
dc.description.abstractMiR-183 expression was quantitated in primary breast tumours, tumour associated normal tissue and breast cancer cell lines using RQ-PCR. Gain of function analysis was performed in breast cancer cells using pre-miR-183 and the effect of miR-183 overexpression on cell viability, proliferation, apoptosis and migration was examined. Customized Taqman Low Density Arrays (TLDA) were used to identify dysregulated genes in breast cancer cells transfected with pre-miR-183.-
dc.description.abstractWe demonstrate that miR-183 is dysregulated in breast cancer and expression correlates with estrogen receptor and HER2/neu receptor expression. Induced overexpression of miR-183 inhibited migration of breast cancer cells. This finding was substantiated by RQ-PCR of mRNA from cells overexpressing miR-183 which showed dysregulation of several migration and invasion related genes. Specifically, the VIL2-coding protein Ezrin was confirmed as a target of miR-183 and downregulation of this protein was confirmed with immunocytochemistry.-
dc.description.abstractThese findings indicate that miR-183 targets VIL2 and may play a central role in the regulation of migration and metastasis in breast cancer. Consequently, this miRNA may present an attractive target for therapeutic intervention.-
dc.language.isoenen
dc.subject.meshApoptosis-
dc.subject.meshBreast Neoplasms-
dc.subject.meshCell Line, Tumor-
dc.subject.meshCell Movement-
dc.subject.meshCytoskeletal Proteins-
dc.subject.meshDown-Regulation-
dc.subject.meshGene Expression Regulation, Neoplastic-
dc.subject.meshHumans-
dc.subject.meshImmunohistochemistry-
dc.subject.meshMicroRNAs-
dc.subject.meshNeoplasm Invasiveness-
dc.subject.meshNeoplasm Metastasis-
dc.subject.meshRNA, Messenger-
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction-
dc.titleDysregulated miR-183 inhibits migration in breast cancer cells.en
dc.typeArticleen
dc.contributor.departmentDepartment of Surgery, National University of Ireland, Galway, Ireland.en
dc.identifier.journalBMC canceren

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