Comparison of two DNA microarrays for detection of plasmid-mediated antimicrobial resistance and virulence factor genes in clinical isolates of Enterobacteriaceae and non-Enterobacteriaceae.

Hdl Handle:
http://hdl.handle.net/10147/124499
Title:
Comparison of two DNA microarrays for detection of plasmid-mediated antimicrobial resistance and virulence factor genes in clinical isolates of Enterobacteriaceae and non-Enterobacteriaceae.
Authors:
Walsh, Fiona; Cooke, Niamh M; Smith, Stephen G; Moran, Gary P; Cooke, Fiona J; Ivens, Alasdair; Wain, John; Rogers, Thomas R
Affiliation:
Department of Clinical Microbiology, Sir Patrick Dun Translational Research Laboratory, School of Medicine, University of Dublin, Trinity College, St James's Hospital Campus, Dublin 8, Ireland. fiona1walsh@gmail.com
Citation:
Comparison of two DNA microarrays for detection of plasmid-mediated antimicrobial resistance and virulence factor genes in clinical isolates of Enterobacteriaceae and non-Enterobacteriaceae. 2010, 35 (6):593-8 Int. J. Antimicrob. Agents
Journal:
International journal of antimicrobial agents
Issue Date:
Jun-2010
URI:
http://hdl.handle.net/10147/124499
DOI:
10.1016/j.ijantimicag.2010.02.011
PubMed ID:
20356716
Abstract:
A DNA microarray was developed to detect plasmid-mediated antimicrobial resistance (AR) and virulence factor (VF) genes in clinical isolates of Enterobacteriaceae and non-Enterobacteriaceae. The array was validated with the following bacterial species: Escherichiacoli (n=17); Klebsiellapneumoniae (n=3); Enterobacter spp. (n=6); Acinetobacter genospecies 3 (n=1); Acinetobacterbaumannii (n=1); Pseudomonasaeruginosa (n=2); and Stenotrophomonasmaltophilia (n=2). The AR gene profiles of these isolates were identified by polymerase chain reaction (PCR). The DNA microarray consisted of 155 and 133 AR and VF gene probes, respectively. Results were compared with the commercially available Identibac AMR-ve Array Tube. Hybridisation results indicated that there was excellent correlation between PCR and array results for AR and VF genes. Genes conferring resistance to each antibiotic class were identified by the DNA array. Unusual resistance genes were also identified, such as bla(SHV-5) in a bla(OXA-23)-positive carbapenem-resistant A. baumannii. The phylogenetic group of each E. coli isolate was verified by the array. These data demonstrate that it is possible to screen simultaneously for all important classes of mobile AR and VF genes in Enterobacteriaceae and non-Enterobacteriaceae whilst also assigning a correct phylogenetic group to E. coli isolates. Therefore, it is feasible to test clinical Gram-negative bacteria for all known AR genes and to provide important information regarding pathogenicity simultaneously.
Item Type:
Article
Language:
en
MeSH:
Bacterial Proteins; Bacteriological Techniques; Drug Resistance, Bacterial; Genes, Bacterial; Gram-Negative Bacteria; Gram-Negative Bacterial Infections; Humans; Oligonucleotide Array Sequence Analysis; Sensitivity and Specificity; Virulence Factors
ISSN:
1872-7913

Full metadata record

DC FieldValue Language
dc.contributor.authorWalsh, Fionaen
dc.contributor.authorCooke, Niamh Men
dc.contributor.authorSmith, Stephen Gen
dc.contributor.authorMoran, Gary Pen
dc.contributor.authorCooke, Fiona Jen
dc.contributor.authorIvens, Alasdairen
dc.contributor.authorWain, Johnen
dc.contributor.authorRogers, Thomas Ren
dc.date.accessioned2011-03-14T15:40:19Z-
dc.date.available2011-03-14T15:40:19Z-
dc.date.issued2010-06-
dc.identifier.citationComparison of two DNA microarrays for detection of plasmid-mediated antimicrobial resistance and virulence factor genes in clinical isolates of Enterobacteriaceae and non-Enterobacteriaceae. 2010, 35 (6):593-8 Int. J. Antimicrob. Agentsen
dc.identifier.issn1872-7913-
dc.identifier.pmid20356716-
dc.identifier.doi10.1016/j.ijantimicag.2010.02.011-
dc.identifier.urihttp://hdl.handle.net/10147/124499-
dc.description.abstractA DNA microarray was developed to detect plasmid-mediated antimicrobial resistance (AR) and virulence factor (VF) genes in clinical isolates of Enterobacteriaceae and non-Enterobacteriaceae. The array was validated with the following bacterial species: Escherichiacoli (n=17); Klebsiellapneumoniae (n=3); Enterobacter spp. (n=6); Acinetobacter genospecies 3 (n=1); Acinetobacterbaumannii (n=1); Pseudomonasaeruginosa (n=2); and Stenotrophomonasmaltophilia (n=2). The AR gene profiles of these isolates were identified by polymerase chain reaction (PCR). The DNA microarray consisted of 155 and 133 AR and VF gene probes, respectively. Results were compared with the commercially available Identibac AMR-ve Array Tube. Hybridisation results indicated that there was excellent correlation between PCR and array results for AR and VF genes. Genes conferring resistance to each antibiotic class were identified by the DNA array. Unusual resistance genes were also identified, such as bla(SHV-5) in a bla(OXA-23)-positive carbapenem-resistant A. baumannii. The phylogenetic group of each E. coli isolate was verified by the array. These data demonstrate that it is possible to screen simultaneously for all important classes of mobile AR and VF genes in Enterobacteriaceae and non-Enterobacteriaceae whilst also assigning a correct phylogenetic group to E. coli isolates. Therefore, it is feasible to test clinical Gram-negative bacteria for all known AR genes and to provide important information regarding pathogenicity simultaneously.-
dc.language.isoenen
dc.subject.meshBacterial Proteins-
dc.subject.meshBacteriological Techniques-
dc.subject.meshDrug Resistance, Bacterial-
dc.subject.meshGenes, Bacterial-
dc.subject.meshGram-Negative Bacteria-
dc.subject.meshGram-Negative Bacterial Infections-
dc.subject.meshHumans-
dc.subject.meshOligonucleotide Array Sequence Analysis-
dc.subject.meshSensitivity and Specificity-
dc.subject.meshVirulence Factors-
dc.titleComparison of two DNA microarrays for detection of plasmid-mediated antimicrobial resistance and virulence factor genes in clinical isolates of Enterobacteriaceae and non-Enterobacteriaceae.en
dc.typeArticleen
dc.contributor.departmentDepartment of Clinical Microbiology, Sir Patrick Dun Translational Research Laboratory, School of Medicine, University of Dublin, Trinity College, St James's Hospital Campus, Dublin 8, Ireland. fiona1walsh@gmail.comen
dc.identifier.journalInternational journal of antimicrobial agentsen
dc.description.provinceLeinster-

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