Enhanced discrimination of highly clonal ST22-methicillin-resistant Staphylococcus aureus IV isolates achieved by combining spa, dru, and pulsed-field gel electrophoresis typing data.

Hdl Handle:
http://hdl.handle.net/10147/124497
Title:
Enhanced discrimination of highly clonal ST22-methicillin-resistant Staphylococcus aureus IV isolates achieved by combining spa, dru, and pulsed-field gel electrophoresis typing data.
Authors:
Shore, Anna C; Rossney, Angela S; Kinnevey, Peter M; Brennan, Orla M; Creamer, Eilish; Sherlock, Orla; Dolan, Anthony; Cunney, Robert; Sullivan, Derek J; Goering, Richard V; Humphreys, Hilary; Coleman, David C
Affiliation:
Microbiology Research Unit, Division of Oral Biosciences, School of Dental Science and Dublin Dental Hospital, University of Dublin, Trinity College Dublin, Dublin 2, Ireland.
Citation:
Enhanced discrimination of highly clonal ST22-methicillin-resistant Staphylococcus aureus IV isolates achieved by combining spa, dru, and pulsed-field gel electrophoresis typing data. 2010, 48 (5):1839-52 J. Clin. Microbiol.
Journal:
Journal of clinical microbiology
Issue Date:
May-2010
URI:
http://hdl.handle.net/10147/124497
DOI:
10.1128/JCM.02155-09
PubMed ID:
20335411
Additional Links:
http://jcm.asm.org/cgi/reprint/48/5/1839
Abstract:
ST22-methicillin-resistant Staphylococcus aureus type IV (ST22-MRSA-IV) is endemic in Irish hospitals and is designated antibiogram-resistogram type-pulsed-field group (AR-PFG) 06-01. Isolates of this highly clonal strain exhibit limited numbers of pulsed-field gel electrophoresis (PFGE) patterns and spa types. This study investigated whether combining PFGE and spa typing with DNA sequencing of the staphylococcal cassette chromosome mec element (SCCmec)-associated direct repeat unit (dru typing) would improve isolate discrimination. A total of 173 MRSA isolates recovered in one Irish hospital during periods in 2007 and 2008 were investigated using antibiogram-resistogram (AR), PFGE, spa, dru, and SCCmec typing. Isolates representative of each of the 17 pulsed-field group 01 (PFG-01) spa types identified underwent multilocus sequence typing, and all isolates were ST22. Ninety-seven percent of isolates (168 of 173) exhibited AR-PFG 06-01 or closely related AR patterns, and 163 of these isolates harbored SCCmec type IVh. The combination of PFGE, spa, and dru typing methods significantly improved discrimination of the 168 PFG-01 isolates, yielding 65 type combinations with a Simpson's index of diversity (SID) of 96.53, compared to (i) pairwise combinations of spa and dru typing, spa and PFGE typing, and dru and PFGE typing, which yielded 37, 44, and 43 type combinations with SIDs of 90.84, 91.00, and 93.57, respectively, or (ii) individual spa, dru, and PFGE typing methods, which yielded 17, 17, and 21 types with SIDs of 66.9, 77.83, and 81.34, respectively. Analysis of epidemiological information for a subset of PFG-01 isolates validated the relationships inferred using combined PFGE, spa, and dru typing data. This approach significantly enhances discrimination of ST22-MRSA-IV isolates and could be applied to epidemiological investigations of other highly clonal MRSA strains.
Item Type:
Article
Language:
en
MeSH:
Bacterial Typing Techniques; Cluster Analysis; DNA Fingerprinting; DNA, Bacterial; Electrophoresis, Gel, Pulsed-Field; Genotype; Humans; Ireland; Methicillin-Resistant Staphylococcus aureus; Microbial Sensitivity Tests; Sensitivity and Specificity; Sequence Analysis, DNA; Staphylococcal Infections; Staphylococcal Protein A
ISSN:
1098-660X

Full metadata record

DC FieldValue Language
dc.contributor.authorShore, Anna Cen
dc.contributor.authorRossney, Angela Sen
dc.contributor.authorKinnevey, Peter Men
dc.contributor.authorBrennan, Orla Men
dc.contributor.authorCreamer, Eilishen
dc.contributor.authorSherlock, Orlaen
dc.contributor.authorDolan, Anthonyen
dc.contributor.authorCunney, Roberten
dc.contributor.authorSullivan, Derek Jen
dc.contributor.authorGoering, Richard Ven
dc.contributor.authorHumphreys, Hilaryen
dc.contributor.authorColeman, David Cen
dc.date.accessioned2011-03-14T15:37:27Z-
dc.date.available2011-03-14T15:37:27Z-
dc.date.issued2010-05-
dc.identifier.citationEnhanced discrimination of highly clonal ST22-methicillin-resistant Staphylococcus aureus IV isolates achieved by combining spa, dru, and pulsed-field gel electrophoresis typing data. 2010, 48 (5):1839-52 J. Clin. Microbiol.en
dc.identifier.issn1098-660X-
dc.identifier.pmid20335411-
dc.identifier.doi10.1128/JCM.02155-09-
dc.identifier.urihttp://hdl.handle.net/10147/124497-
dc.description.abstractST22-methicillin-resistant Staphylococcus aureus type IV (ST22-MRSA-IV) is endemic in Irish hospitals and is designated antibiogram-resistogram type-pulsed-field group (AR-PFG) 06-01. Isolates of this highly clonal strain exhibit limited numbers of pulsed-field gel electrophoresis (PFGE) patterns and spa types. This study investigated whether combining PFGE and spa typing with DNA sequencing of the staphylococcal cassette chromosome mec element (SCCmec)-associated direct repeat unit (dru typing) would improve isolate discrimination. A total of 173 MRSA isolates recovered in one Irish hospital during periods in 2007 and 2008 were investigated using antibiogram-resistogram (AR), PFGE, spa, dru, and SCCmec typing. Isolates representative of each of the 17 pulsed-field group 01 (PFG-01) spa types identified underwent multilocus sequence typing, and all isolates were ST22. Ninety-seven percent of isolates (168 of 173) exhibited AR-PFG 06-01 or closely related AR patterns, and 163 of these isolates harbored SCCmec type IVh. The combination of PFGE, spa, and dru typing methods significantly improved discrimination of the 168 PFG-01 isolates, yielding 65 type combinations with a Simpson's index of diversity (SID) of 96.53, compared to (i) pairwise combinations of spa and dru typing, spa and PFGE typing, and dru and PFGE typing, which yielded 37, 44, and 43 type combinations with SIDs of 90.84, 91.00, and 93.57, respectively, or (ii) individual spa, dru, and PFGE typing methods, which yielded 17, 17, and 21 types with SIDs of 66.9, 77.83, and 81.34, respectively. Analysis of epidemiological information for a subset of PFG-01 isolates validated the relationships inferred using combined PFGE, spa, and dru typing data. This approach significantly enhances discrimination of ST22-MRSA-IV isolates and could be applied to epidemiological investigations of other highly clonal MRSA strains.-
dc.language.isoenen
dc.relation.urlhttp://jcm.asm.org/cgi/reprint/48/5/1839-
dc.subject.meshBacterial Typing Techniques-
dc.subject.meshCluster Analysis-
dc.subject.meshDNA Fingerprinting-
dc.subject.meshDNA, Bacterial-
dc.subject.meshElectrophoresis, Gel, Pulsed-Field-
dc.subject.meshGenotype-
dc.subject.meshHumans-
dc.subject.meshIreland-
dc.subject.meshMethicillin-Resistant Staphylococcus aureus-
dc.subject.meshMicrobial Sensitivity Tests-
dc.subject.meshSensitivity and Specificity-
dc.subject.meshSequence Analysis, DNA-
dc.subject.meshStaphylococcal Infections-
dc.subject.meshStaphylococcal Protein A-
dc.titleEnhanced discrimination of highly clonal ST22-methicillin-resistant Staphylococcus aureus IV isolates achieved by combining spa, dru, and pulsed-field gel electrophoresis typing data.en
dc.typeArticleen
dc.contributor.departmentMicrobiology Research Unit, Division of Oral Biosciences, School of Dental Science and Dublin Dental Hospital, University of Dublin, Trinity College Dublin, Dublin 2, Ireland.en
dc.identifier.journalJournal of clinical microbiologyen
dc.description.provinceLeinster-

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