Comparative genomics of the fungal pathogens Candida dubliniensis and Candida albicans.

Hdl Handle:
http://hdl.handle.net/10147/124485
Title:
Comparative genomics of the fungal pathogens Candida dubliniensis and Candida albicans.
Authors:
Jackson, Andrew P; Gamble, John A; Yeomans, Tim; Moran, Gary P; Saunders, David; Harris, David; Aslett, Martin; Barrell, Jamie F; Butler, Geraldine; Citiulo, Francesco; Coleman, David C; de Groot, Piet W J; Goodwin, Tim J; Quail, Michael A; McQuillan, Jacqueline; Munro, Carol A; Pain, Arnab; Poulter, Russell T; Rajandream, Marie-Adèle; Renauld, Hubert; Spiering, Martin J; Tivey, Adrian; Gow, Neil A R; Barrell, Barclay; Sullivan, Derek J; Berriman, Matthew
Affiliation:
Pathogen Genomics Group, Wellcome Trust Sanger Institute, Cambridge, United Kingdom. aj4@sanger.ac.uk
Citation:
Comparative genomics of the fungal pathogens Candida dubliniensis and Candida albicans. 2009, 19 (12):2231-44 Genome Res.
Journal:
Genome research
Issue Date:
Dec-2009
URI:
http://hdl.handle.net/10147/124485
DOI:
10.1101/gr.097501.109
PubMed ID:
19745113
Abstract:
Candida dubliniensis is the closest known relative of Candida albicans, the most pathogenic yeast species in humans. However, despite both species sharing many phenotypic characteristics, including the ability to form true hyphae, C. dubliniensis is a significantly less virulent and less versatile pathogen. Therefore, to identify C. albicans-specific genes that may be responsible for an increased capacity to cause disease, we have sequenced the C. dubliniensis genome and compared it with the known C. albicans genome sequence. Although the two genome sequences are highly similar and synteny is conserved throughout, 168 species-specific genes are identified, including some encoding known hyphal-specific virulence factors, such as the aspartyl proteinases Sap4 and Sap5 and the proposed invasin Als3. Among the 115 pseudogenes confirmed in C. dubliniensis are orthologs of several filamentous growth regulator (FGR) genes that also have suspected roles in pathogenesis. However, the principal differences in genomic repertoire concern expansion of the TLO gene family of putative transcription factors and the IFA family of putative transmembrane proteins in C. albicans, which represent novel candidate virulence-associated factors. The results suggest that the recent evolutionary histories of C. albicans and C. dubliniensis are quite different. While gene families instrumental in pathogenesis have been elaborated in C. albicans, C. dubliniensis has lost genomic capacity and key pathogenic functions. This could explain why C. albicans is a more potent pathogen in humans than C. dubliniensis.
Item Type:
Article
Language:
en
MeSH:
Candida; Candida albicans; Fungal Proteins; Gene Order; Genome, Fungal; Genomics; Humans; Hyphae; Membrane Proteins; Molecular Sequence Data; Phylogeny; Sequence Analysis, DNA; Species Specificity; Synteny; Transcription Factors; Virulence; Virulence Factors
ISSN:
1549-5469

Full metadata record

DC FieldValue Language
dc.contributor.authorJackson, Andrew Pen
dc.contributor.authorGamble, John Aen
dc.contributor.authorYeomans, Timen
dc.contributor.authorMoran, Gary Pen
dc.contributor.authorSaunders, Daviden
dc.contributor.authorHarris, Daviden
dc.contributor.authorAslett, Martinen
dc.contributor.authorBarrell, Jamie Fen
dc.contributor.authorButler, Geraldineen
dc.contributor.authorCitiulo, Francescoen
dc.contributor.authorColeman, David Cen
dc.contributor.authorde Groot, Piet W Jen
dc.contributor.authorGoodwin, Tim Jen
dc.contributor.authorQuail, Michael Aen
dc.contributor.authorMcQuillan, Jacquelineen
dc.contributor.authorMunro, Carol Aen
dc.contributor.authorPain, Arnaben
dc.contributor.authorPoulter, Russell Ten
dc.contributor.authorRajandream, Marie-Adèleen
dc.contributor.authorRenauld, Huberten
dc.contributor.authorSpiering, Martin Jen
dc.contributor.authorTivey, Adrianen
dc.contributor.authorGow, Neil A Ren
dc.contributor.authorBarrell, Barclayen
dc.contributor.authorSullivan, Derek Jen
dc.contributor.authorBerriman, Matthewen
dc.date.accessioned2011-03-14T12:57:16Z-
dc.date.available2011-03-14T12:57:16Z-
dc.date.issued2009-12-
dc.identifier.citationComparative genomics of the fungal pathogens Candida dubliniensis and Candida albicans. 2009, 19 (12):2231-44 Genome Res.en
dc.identifier.issn1549-5469-
dc.identifier.pmid19745113-
dc.identifier.doi10.1101/gr.097501.109-
dc.identifier.urihttp://hdl.handle.net/10147/124485-
dc.description.abstractCandida dubliniensis is the closest known relative of Candida albicans, the most pathogenic yeast species in humans. However, despite both species sharing many phenotypic characteristics, including the ability to form true hyphae, C. dubliniensis is a significantly less virulent and less versatile pathogen. Therefore, to identify C. albicans-specific genes that may be responsible for an increased capacity to cause disease, we have sequenced the C. dubliniensis genome and compared it with the known C. albicans genome sequence. Although the two genome sequences are highly similar and synteny is conserved throughout, 168 species-specific genes are identified, including some encoding known hyphal-specific virulence factors, such as the aspartyl proteinases Sap4 and Sap5 and the proposed invasin Als3. Among the 115 pseudogenes confirmed in C. dubliniensis are orthologs of several filamentous growth regulator (FGR) genes that also have suspected roles in pathogenesis. However, the principal differences in genomic repertoire concern expansion of the TLO gene family of putative transcription factors and the IFA family of putative transmembrane proteins in C. albicans, which represent novel candidate virulence-associated factors. The results suggest that the recent evolutionary histories of C. albicans and C. dubliniensis are quite different. While gene families instrumental in pathogenesis have been elaborated in C. albicans, C. dubliniensis has lost genomic capacity and key pathogenic functions. This could explain why C. albicans is a more potent pathogen in humans than C. dubliniensis.-
dc.language.isoenen
dc.subject.meshCandida-
dc.subject.meshCandida albicans-
dc.subject.meshFungal Proteins-
dc.subject.meshGene Order-
dc.subject.meshGenome, Fungal-
dc.subject.meshGenomics-
dc.subject.meshHumans-
dc.subject.meshHyphae-
dc.subject.meshMembrane Proteins-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshPhylogeny-
dc.subject.meshSequence Analysis, DNA-
dc.subject.meshSpecies Specificity-
dc.subject.meshSynteny-
dc.subject.meshTranscription Factors-
dc.subject.meshVirulence-
dc.subject.meshVirulence Factors-
dc.titleComparative genomics of the fungal pathogens Candida dubliniensis and Candida albicans.en
dc.typeArticleen
dc.contributor.departmentPathogen Genomics Group, Wellcome Trust Sanger Institute, Cambridge, United Kingdom. aj4@sanger.ac.uken
dc.identifier.journalGenome researchen
dc.description.provinceLeinster-

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