Comparative genomics of the fungal pathogens Candida dubliniensis and Candida albicans.
Authors
Jackson, Andrew PGamble, John A
Yeomans, Tim
Moran, Gary P
Saunders, David
Harris, David
Aslett, Martin
Barrell, Jamie F
Butler, Geraldine
Citiulo, Francesco
Coleman, David C
de Groot, Piet W J
Goodwin, Tim J
Quail, Michael A
McQuillan, Jacqueline
Munro, Carol A
Pain, Arnab
Poulter, Russell T
Rajandream, Marie-Adèle
Renauld, Hubert
Spiering, Martin J
Tivey, Adrian
Gow, Neil A R
Barrell, Barclay
Sullivan, Derek J
Berriman, Matthew
Affiliation
Pathogen Genomics Group, Wellcome Trust Sanger Institute, Cambridge, United Kingdom. aj4@sanger.ac.ukIssue Date
2009-12MeSH
CandidaCandida albicans
Fungal Proteins
Gene Order
Genome, Fungal
Genomics
Humans
Hyphae
Membrane Proteins
Molecular Sequence Data
Phylogeny
Sequence Analysis, DNA
Species Specificity
Synteny
Transcription Factors
Virulence
Virulence Factors
Metadata
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Comparative genomics of the fungal pathogens Candida dubliniensis and Candida albicans. 2009, 19 (12):2231-44 Genome Res.Journal
Genome researchDOI
10.1101/gr.097501.109PubMed ID
19745113Abstract
Candida dubliniensis is the closest known relative of Candida albicans, the most pathogenic yeast species in humans. However, despite both species sharing many phenotypic characteristics, including the ability to form true hyphae, C. dubliniensis is a significantly less virulent and less versatile pathogen. Therefore, to identify C. albicans-specific genes that may be responsible for an increased capacity to cause disease, we have sequenced the C. dubliniensis genome and compared it with the known C. albicans genome sequence. Although the two genome sequences are highly similar and synteny is conserved throughout, 168 species-specific genes are identified, including some encoding known hyphal-specific virulence factors, such as the aspartyl proteinases Sap4 and Sap5 and the proposed invasin Als3. Among the 115 pseudogenes confirmed in C. dubliniensis are orthologs of several filamentous growth regulator (FGR) genes that also have suspected roles in pathogenesis. However, the principal differences in genomic repertoire concern expansion of the TLO gene family of putative transcription factors and the IFA family of putative transmembrane proteins in C. albicans, which represent novel candidate virulence-associated factors. The results suggest that the recent evolutionary histories of C. albicans and C. dubliniensis are quite different. While gene families instrumental in pathogenesis have been elaborated in C. albicans, C. dubliniensis has lost genomic capacity and key pathogenic functions. This could explain why C. albicans is a more potent pathogen in humans than C. dubliniensis.Item Type
ArticleLanguage
enISSN
1549-5469ae974a485f413a2113503eed53cd6c53
10.1101/gr.097501.109
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