Purification and germination of Candida albicans and Candida dubliniensis chlamydospores cultured in liquid media.

Hdl Handle:
http://hdl.handle.net/10147/124474
Title:
Purification and germination of Candida albicans and Candida dubliniensis chlamydospores cultured in liquid media.
Authors:
Citiulo, Francesco; Moran, Gary P; Coleman, David C; Sullivan, Derek J
Affiliation:
Microbiology Research Unit, Division of Oral Biosciences, Dublin Dental School and Hospital, Trinity College Dublin, University of Dublin, Dublin 2, Ireland.
Citation:
Purification and germination of Candida albicans and Candida dubliniensis chlamydospores cultured in liquid media. 2009, 9 (7):1051-60 FEMS Yeast Res.
Journal:
FEMS yeast research
Issue Date:
Oct-2009
URI:
http://hdl.handle.net/10147/124474
DOI:
10.1111/j.1567-1364.2009.00533.x
PubMed ID:
19538507
Abstract:
Candida albicans and Candida dubliniensis are the only Candida sp. that have been observed to produce chlamydospores. The function of these large, thick-walled cells is currently unknown. In this report, we describe the production and purification of chlamydospores from these species in defined liquid media. Staining with the fluorescent dye FUN-1 indicated that chlamydospores are metabolically active cells, but that metabolic activity is undetectable in chlamydospores that are >30 days old. However, 5-15-day-old chlamydospores could be induced to produce daughter chlamydospores, blastospores, pseudohyphae and true hyphae depending on the incubation conditions used. Chlamydospores that were preinduced to germinate were also observed to escape from murine macrophages following phagocytosis, suggesting that these structures may be viable in vivo. Mycelium-attached and purified chlamydospores rapidly lost their viability in water and when subjected to dry stress, suggesting that they are unlikely to act as long-term storage structures. Instead, our data suggest that chlamydospores represent an alternative specialized form of growth by C. albicans and C. dubliniensis.
Item Type:
Article
Language:
en
MeSH:
Animals; Candida; Culture Media; Fluorescent Dyes; Macrophages; Mice; Microbial Viability; Microscopy; Microscopy, Electron, Scanning; Mycology; Spores, Fungal
ISSN:
1567-1364

Full metadata record

DC FieldValue Language
dc.contributor.authorCitiulo, Francescoen
dc.contributor.authorMoran, Gary Pen
dc.contributor.authorColeman, David Cen
dc.contributor.authorSullivan, Derek Jen
dc.date.accessioned2011-03-14T12:26:48Z-
dc.date.available2011-03-14T12:26:48Z-
dc.date.issued2009-10-
dc.identifier.citationPurification and germination of Candida albicans and Candida dubliniensis chlamydospores cultured in liquid media. 2009, 9 (7):1051-60 FEMS Yeast Res.en
dc.identifier.issn1567-1364-
dc.identifier.pmid19538507-
dc.identifier.doi10.1111/j.1567-1364.2009.00533.x-
dc.identifier.urihttp://hdl.handle.net/10147/124474-
dc.description.abstractCandida albicans and Candida dubliniensis are the only Candida sp. that have been observed to produce chlamydospores. The function of these large, thick-walled cells is currently unknown. In this report, we describe the production and purification of chlamydospores from these species in defined liquid media. Staining with the fluorescent dye FUN-1 indicated that chlamydospores are metabolically active cells, but that metabolic activity is undetectable in chlamydospores that are >30 days old. However, 5-15-day-old chlamydospores could be induced to produce daughter chlamydospores, blastospores, pseudohyphae and true hyphae depending on the incubation conditions used. Chlamydospores that were preinduced to germinate were also observed to escape from murine macrophages following phagocytosis, suggesting that these structures may be viable in vivo. Mycelium-attached and purified chlamydospores rapidly lost their viability in water and when subjected to dry stress, suggesting that they are unlikely to act as long-term storage structures. Instead, our data suggest that chlamydospores represent an alternative specialized form of growth by C. albicans and C. dubliniensis.-
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCandida-
dc.subject.meshCulture Media-
dc.subject.meshFluorescent Dyes-
dc.subject.meshMacrophages-
dc.subject.meshMice-
dc.subject.meshMicrobial Viability-
dc.subject.meshMicroscopy-
dc.subject.meshMicroscopy, Electron, Scanning-
dc.subject.meshMycology-
dc.subject.meshSpores, Fungal-
dc.titlePurification and germination of Candida albicans and Candida dubliniensis chlamydospores cultured in liquid media.en
dc.typeArticleen
dc.contributor.departmentMicrobiology Research Unit, Division of Oral Biosciences, Dublin Dental School and Hospital, Trinity College Dublin, University of Dublin, Dublin 2, Ireland.en
dc.identifier.journalFEMS yeast researchen
dc.description.provinceLeinster-

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