Lack of cytotoxicity by Trustwater Ecasol™ used to maintain good quality dental unit waterline output water in keratinocyte monolayer and reconstituted human oral epithelial tissue models.

Hdl Handle:
http://hdl.handle.net/10147/124111
Title:
Lack of cytotoxicity by Trustwater Ecasol™ used to maintain good quality dental unit waterline output water in keratinocyte monolayer and reconstituted human oral epithelial tissue models.
Authors:
Boyle, M A; O'Donnell, M J; Russell, R J; Coleman, D C
Affiliation:
Microbiology Research Unit, Division of Oral Biosciences, Dublin Dental School & Hospital, University of Dublin, Trinity College Dublin, Lincoln Place, Dublin 2, Ireland.
Citation:
Lack of cytotoxicity by Trustwater Ecasol™ used to maintain good quality dental unit waterline output water in keratinocyte monolayer and reconstituted human oral epithelial tissue models. 2010, 38 (11):930-40 J Dent
Journal:
Journal of dentistry
Issue Date:
Nov-2010
URI:
http://hdl.handle.net/10147/124111
DOI:
10.1016/j.jdent.2010.08.005
PubMed ID:
20713118
Abstract:
We previously showed that residual treatment of dental chair unit (DCU) supply water using the electrochemically-activated solution Trustwater Ecasol™ (2.5 ppm) provided an effective long-term solution to the problem of dental unit waterline (DUWL) biofilm resulting in DUWL output water quality consistently superior to potable water.; To investigate the cytoxicity of Ecasol using cultured keratinocyte monolayers and reconstituted human oral epithelial (RHE) tissue and to extend the study of Ecasol's effectiveness in maintaining the microbiological quality of DUWL output water.; TR146 human keratinocyte monolayers and RHE tissues were exposed to Ecasol (2.5-100 ppm) for 1h periods after removal of growth medium and washing with phosphate-buffered saline (PBS). Experiments were repeated using Ecasol that had been exposed for 30 min to 1-2mg/mL bovine serum albumin (BSA), equivalent to protein concentrations in saliva. To quantitatively determine cytotoxic effects on monolayers following Ecasol exposure, the Alamar Blue proliferation assay (assesses cell viability) and the Trypan Blue exclusion assay (assesses plasma membrane integrity), were used. Cytotoxicity effects on RHE tissues were assessed by the Alamar Blue assay and by histopathology.; Ecasol at >5.0 ppm resulted in significant (P<0.001) cytotoxicity to keratinocyte monolayers following a 1h exposure. These effects, however, were completely negated by BSA pretreatment of Ecasol. No cytotoxicity was observed in the more complex RHE tissue at any of the Ecasol concentrations tested. In a 60-week study of 10 DCUs, tested weekly, the average density of aerobic heterotrophic bacteria in Ecasol-treated (2.5 ppm) DCU supply water was <1cfu/mL and in DUWL output water was 6.5cfu/mL.; Ecasol present as a residual disinfectant in DUWL output water is very unlikely to have adverse effects on human oral tissues at levels effective in maintaining DUWL output water quality at better than potable standard water quality.
Item Type:
Article
Language:
en
MeSH:
Bacteria, Aerobic; Bacterial Load; Biofilms; Buffers; Cell Culture Techniques; Cell Line, Tumor; Cell Membrane; Cell Proliferation; Cell Survival; Coloring Agents; Dental Disinfectants; Dental Equipment; Epithelial Cells; Humans; Hypochlorous Acid; Indicators and Reagents; Keratinocytes; Mouth Mucosa; Oxazines; Phosphates; Serum Albumin, Bovine; Sodium Chloride; Time Factors; Tissue Scaffolds; Trypan Blue; Water Microbiology; Water Supply; Xanthenes
ISSN:
1879-176X

Full metadata record

DC FieldValue Language
dc.contributor.authorBoyle, M Aen
dc.contributor.authorO'Donnell, M Jen
dc.contributor.authorRussell, R Jen
dc.contributor.authorColeman, D Cen
dc.date.accessioned2011-03-10T12:47:09Z-
dc.date.available2011-03-10T12:47:09Z-
dc.date.issued2010-11-
dc.identifier.citationLack of cytotoxicity by Trustwater Ecasol™ used to maintain good quality dental unit waterline output water in keratinocyte monolayer and reconstituted human oral epithelial tissue models. 2010, 38 (11):930-40 J Denten
dc.identifier.issn1879-176X-
dc.identifier.pmid20713118-
dc.identifier.doi10.1016/j.jdent.2010.08.005-
dc.identifier.urihttp://hdl.handle.net/10147/124111-
dc.description.abstractWe previously showed that residual treatment of dental chair unit (DCU) supply water using the electrochemically-activated solution Trustwater Ecasol™ (2.5 ppm) provided an effective long-term solution to the problem of dental unit waterline (DUWL) biofilm resulting in DUWL output water quality consistently superior to potable water.-
dc.description.abstractTo investigate the cytoxicity of Ecasol using cultured keratinocyte monolayers and reconstituted human oral epithelial (RHE) tissue and to extend the study of Ecasol's effectiveness in maintaining the microbiological quality of DUWL output water.-
dc.description.abstractTR146 human keratinocyte monolayers and RHE tissues were exposed to Ecasol (2.5-100 ppm) for 1h periods after removal of growth medium and washing with phosphate-buffered saline (PBS). Experiments were repeated using Ecasol that had been exposed for 30 min to 1-2mg/mL bovine serum albumin (BSA), equivalent to protein concentrations in saliva. To quantitatively determine cytotoxic effects on monolayers following Ecasol exposure, the Alamar Blue proliferation assay (assesses cell viability) and the Trypan Blue exclusion assay (assesses plasma membrane integrity), were used. Cytotoxicity effects on RHE tissues were assessed by the Alamar Blue assay and by histopathology.-
dc.description.abstractEcasol at >5.0 ppm resulted in significant (P<0.001) cytotoxicity to keratinocyte monolayers following a 1h exposure. These effects, however, were completely negated by BSA pretreatment of Ecasol. No cytotoxicity was observed in the more complex RHE tissue at any of the Ecasol concentrations tested. In a 60-week study of 10 DCUs, tested weekly, the average density of aerobic heterotrophic bacteria in Ecasol-treated (2.5 ppm) DCU supply water was <1cfu/mL and in DUWL output water was 6.5cfu/mL.-
dc.description.abstractEcasol present as a residual disinfectant in DUWL output water is very unlikely to have adverse effects on human oral tissues at levels effective in maintaining DUWL output water quality at better than potable standard water quality.-
dc.language.isoenen
dc.subject.meshBacteria, Aerobic-
dc.subject.meshBacterial Load-
dc.subject.meshBiofilms-
dc.subject.meshBuffers-
dc.subject.meshCell Culture Techniques-
dc.subject.meshCell Line, Tumor-
dc.subject.meshCell Membrane-
dc.subject.meshCell Proliferation-
dc.subject.meshCell Survival-
dc.subject.meshColoring Agents-
dc.subject.meshDental Disinfectants-
dc.subject.meshDental Equipment-
dc.subject.meshEpithelial Cells-
dc.subject.meshHumans-
dc.subject.meshHypochlorous Acid-
dc.subject.meshIndicators and Reagents-
dc.subject.meshKeratinocytes-
dc.subject.meshMouth Mucosa-
dc.subject.meshOxazines-
dc.subject.meshPhosphates-
dc.subject.meshSerum Albumin, Bovine-
dc.subject.meshSodium Chloride-
dc.subject.meshTime Factors-
dc.subject.meshTissue Scaffolds-
dc.subject.meshTrypan Blue-
dc.subject.meshWater Microbiology-
dc.subject.meshWater Supply-
dc.subject.meshXanthenes-
dc.titleLack of cytotoxicity by Trustwater Ecasol™ used to maintain good quality dental unit waterline output water in keratinocyte monolayer and reconstituted human oral epithelial tissue models.en
dc.typeArticleen
dc.contributor.departmentMicrobiology Research Unit, Division of Oral Biosciences, Dublin Dental School & Hospital, University of Dublin, Trinity College Dublin, Lincoln Place, Dublin 2, Ireland.en
dc.identifier.journalJournal of dentistryen
dc.description.provinceLeinster-
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