The expression of genes involved in the ergosterol biosynthesis pathway in Candida albicans and Candida dubliniensis biofilms exposed to fluconazole.

Hdl Handle:
http://hdl.handle.net/10147/124015
Title:
The expression of genes involved in the ergosterol biosynthesis pathway in Candida albicans and Candida dubliniensis biofilms exposed to fluconazole.
Authors:
Borecká-Melkusová, Silvia; Moran, Gary P; Sullivan, Derek J; Kucharíková, Sona; Chorvát, Dusan; Bujdáková, Helena
Affiliation:
Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia. borecka@fns.uniba.sk
Citation:
The expression of genes involved in the ergosterol biosynthesis pathway in Candida albicans and Candida dubliniensis biofilms exposed to fluconazole. 2009, 52 (2):118-28 Mycoses
Journal:
Mycoses
Issue Date:
Mar-2009
URI:
http://hdl.handle.net/10147/124015
DOI:
10.1111/j.1439-0507.2008.01550.x
PubMed ID:
18627475
Abstract:
The expression of the ERG1, ERG3, ERG7, ERG9, ERG11 and ERG25 genes in response to incubation with fluconazole and biofilm formation was investigated using reverse-transcription PCR and real-time PCR in Candida albicans and Candida dubliniensis clinical isolates. The viability of biofilm was measured using an 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction assay and confocal scanning laser microscopy (CSLM). Expression of the ERG11 gene was found to be low or moderate and it was regulated by fluconazole addition more so than by biofilm formation. Very low or non-detectable expression of ERG1, ERG7 and ERG25 genes was detected in C. albicans. The expression of the ERG9 increased in the presence of fluconazole in some isolates. Following incubation with fluconazole, formation of biofilm by C. dubliniensis was coupled with up-regulation of the ERG3 and ERG25 genes as have been observed previously in C. albicans. Planktonic cells of both Candida species released from biofilm displayed similar resistance mechanisms to fluconazole like attached cells. The XTT reduction assay and CSLM revealed that although incubation with fluconazole decreased the biofilm thickness, these were still comprised metabolically active cells able to disseminate and produce biofilm. Our data indicate that biofilm represents a highly adapted community reflecting the individuality of clinical isolates.
Item Type:
Article
Language:
en
MeSH:
Antifungal Agents; Biofilms; Candida; Candida albicans; Drug Resistance, Fungal; Ergosterol; Fluconazole; Fungal Proteins; Gene Expression Regulation, Fungal; Humans; Microbial Sensitivity Tests
ISSN:
1439-0507

Full metadata record

DC FieldValue Language
dc.contributor.authorBorecká-Melkusová, Silviaen
dc.contributor.authorMoran, Gary Pen
dc.contributor.authorSullivan, Derek Jen
dc.contributor.authorKucharíková, Sonaen
dc.contributor.authorChorvát, Dusanen
dc.contributor.authorBujdáková, Helenaen
dc.date.accessioned2011-03-09T11:57:28Z-
dc.date.available2011-03-09T11:57:28Z-
dc.date.issued2009-03-
dc.identifier.citationThe expression of genes involved in the ergosterol biosynthesis pathway in Candida albicans and Candida dubliniensis biofilms exposed to fluconazole. 2009, 52 (2):118-28 Mycosesen
dc.identifier.issn1439-0507-
dc.identifier.pmid18627475-
dc.identifier.doi10.1111/j.1439-0507.2008.01550.x-
dc.identifier.urihttp://hdl.handle.net/10147/124015-
dc.description.abstractThe expression of the ERG1, ERG3, ERG7, ERG9, ERG11 and ERG25 genes in response to incubation with fluconazole and biofilm formation was investigated using reverse-transcription PCR and real-time PCR in Candida albicans and Candida dubliniensis clinical isolates. The viability of biofilm was measured using an 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction assay and confocal scanning laser microscopy (CSLM). Expression of the ERG11 gene was found to be low or moderate and it was regulated by fluconazole addition more so than by biofilm formation. Very low or non-detectable expression of ERG1, ERG7 and ERG25 genes was detected in C. albicans. The expression of the ERG9 increased in the presence of fluconazole in some isolates. Following incubation with fluconazole, formation of biofilm by C. dubliniensis was coupled with up-regulation of the ERG3 and ERG25 genes as have been observed previously in C. albicans. Planktonic cells of both Candida species released from biofilm displayed similar resistance mechanisms to fluconazole like attached cells. The XTT reduction assay and CSLM revealed that although incubation with fluconazole decreased the biofilm thickness, these were still comprised metabolically active cells able to disseminate and produce biofilm. Our data indicate that biofilm represents a highly adapted community reflecting the individuality of clinical isolates.-
dc.language.isoenen
dc.subject.meshAntifungal Agents-
dc.subject.meshBiofilms-
dc.subject.meshCandida-
dc.subject.meshCandida albicans-
dc.subject.meshDrug Resistance, Fungal-
dc.subject.meshErgosterol-
dc.subject.meshFluconazole-
dc.subject.meshFungal Proteins-
dc.subject.meshGene Expression Regulation, Fungal-
dc.subject.meshHumans-
dc.subject.meshMicrobial Sensitivity Tests-
dc.titleThe expression of genes involved in the ergosterol biosynthesis pathway in Candida albicans and Candida dubliniensis biofilms exposed to fluconazole.en
dc.typeArticleen
dc.contributor.departmentDepartment of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia. borecka@fns.uniba.sken
dc.identifier.journalMycosesen
dc.description.provinceLeinster-
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