Differential filamentation of Candida albicans and Candida dubliniensis Is governed by nutrient regulation of UME6 expression.

Hdl Handle:
http://hdl.handle.net/10147/123332
Title:
Differential filamentation of Candida albicans and Candida dubliniensis Is governed by nutrient regulation of UME6 expression.
Authors:
O'Connor, Leanne; Caplice, Nicole; Coleman, David C; Sullivan, Derek J; Moran, Gary P
Affiliation:
Microbiology Research Unit, Division of Oral Biosciences, Dublin Dental School and Hospital, Trinity College Dublin, University of Dublin, Dublin 2, Republic of Ireland. gpmoran@dental.tcd.ie.
Citation:
Differential filamentation of Candida albicans and Candida dubliniensis Is governed by nutrient regulation of UME6 expression. 2010, 9 (9):1383-97 Eukaryotic Cell
Journal:
Eukaryotic cell
Issue Date:
Sep-2010
URI:
http://hdl.handle.net/10147/123332
DOI:
10.1128/EC.00042-10
PubMed ID:
20639413
Additional Links:
http://ec.asm.org/cgi/content/full/9/9/1383
Abstract:
Candida dubliniensis is closely related to Candida albicans; however, it is responsible for fewer infections in humans and is less virulent in animal models of infection. C. dubliniensis forms fewer hyphae in vivo, and this may contribute to its reduced virulence. In this study we show that, unlike C. albicans, C. dubliniensis fails to form hyphae in yeast extract-peptone-dextrose (YPD) medium supplemented with 10% (vol/vol) fetal calf serum (YPDS medium). However, C. dubliniensis filaments in water plus 10% (vol/vol) fetal calf serum (WS), and this filamentation is inhibited by the addition of peptone and glucose. Repression of filamentation in YPDS medium could be partly overcome by preculture in synthetic Lee's medium. Unlike C. albicans, inoculation of C. dubliniensis in YPDS medium did not result in increased UME6 transcription. However, >100-fold induction of UME6 was observed when C. dubliniensis was inoculated in nutrient-poor WS medium. The addition of increasing concentrations of peptone to WS medium had a dose-dependent effect on reducing UME6 expression. Transcript profiling of C. dubliniensis hyphae in WS medium identified a starvation response involving expression of genes in the glyoxylate cycle and fatty acid oxidation. In addition, a core, shared transcriptional response with C. albicans could be identified, including expression of virulence-associated genes including SAP456, SAP7, HWP1, and SOD5. Preculture in nutrient-limiting medium enhanced adherence of C. dubliniensis, epithelial invasion, and survival following coculture with murine macrophages. In conclusion, C. albicans, unlike C. dubliniensis, appears to form hyphae in liquid medium regardless of nutrient availability, which may account for its increased capacity to cause disease in humans.
Language:
en
MeSH:
Animals; Candida; Candida albicans; Candidiasis; Cell Line; Culture Media; Fungal Proteins; Gene Expression Regulation, Developmental; Gene Expression Regulation, Fungal; Humans; Hyphae; Mice; Repressor Proteins; Virulence
ISSN:
1535-9786

Full metadata record

DC FieldValue Language
dc.contributor.authorO'Connor, Leanneen
dc.contributor.authorCaplice, Nicoleen
dc.contributor.authorColeman, David Cen
dc.contributor.authorSullivan, Derek Jen
dc.contributor.authorMoran, Gary Pen
dc.date.accessioned2011-03-02T12:44:39Z-
dc.date.available2011-03-02T12:44:39Z-
dc.date.issued2010-09-
dc.identifier.citationDifferential filamentation of Candida albicans and Candida dubliniensis Is governed by nutrient regulation of UME6 expression. 2010, 9 (9):1383-97 Eukaryotic Cellen
dc.identifier.issn1535-9786-
dc.identifier.pmid20639413-
dc.identifier.doi10.1128/EC.00042-10-
dc.identifier.urihttp://hdl.handle.net/10147/123332-
dc.description.abstractCandida dubliniensis is closely related to Candida albicans; however, it is responsible for fewer infections in humans and is less virulent in animal models of infection. C. dubliniensis forms fewer hyphae in vivo, and this may contribute to its reduced virulence. In this study we show that, unlike C. albicans, C. dubliniensis fails to form hyphae in yeast extract-peptone-dextrose (YPD) medium supplemented with 10% (vol/vol) fetal calf serum (YPDS medium). However, C. dubliniensis filaments in water plus 10% (vol/vol) fetal calf serum (WS), and this filamentation is inhibited by the addition of peptone and glucose. Repression of filamentation in YPDS medium could be partly overcome by preculture in synthetic Lee's medium. Unlike C. albicans, inoculation of C. dubliniensis in YPDS medium did not result in increased UME6 transcription. However, >100-fold induction of UME6 was observed when C. dubliniensis was inoculated in nutrient-poor WS medium. The addition of increasing concentrations of peptone to WS medium had a dose-dependent effect on reducing UME6 expression. Transcript profiling of C. dubliniensis hyphae in WS medium identified a starvation response involving expression of genes in the glyoxylate cycle and fatty acid oxidation. In addition, a core, shared transcriptional response with C. albicans could be identified, including expression of virulence-associated genes including SAP456, SAP7, HWP1, and SOD5. Preculture in nutrient-limiting medium enhanced adherence of C. dubliniensis, epithelial invasion, and survival following coculture with murine macrophages. In conclusion, C. albicans, unlike C. dubliniensis, appears to form hyphae in liquid medium regardless of nutrient availability, which may account for its increased capacity to cause disease in humans.-
dc.language.isoenen
dc.relation.urlhttp://ec.asm.org/cgi/content/full/9/9/1383en
dc.subject.meshAnimals-
dc.subject.meshCandida-
dc.subject.meshCandida albicans-
dc.subject.meshCandidiasis-
dc.subject.meshCell Line-
dc.subject.meshCulture Media-
dc.subject.meshFungal Proteins-
dc.subject.meshGene Expression Regulation, Developmental-
dc.subject.meshGene Expression Regulation, Fungal-
dc.subject.meshHumans-
dc.subject.meshHyphae-
dc.subject.meshMice-
dc.subject.meshRepressor Proteins-
dc.subject.meshVirulence-
dc.titleDifferential filamentation of Candida albicans and Candida dubliniensis Is governed by nutrient regulation of UME6 expression.en
dc.contributor.departmentMicrobiology Research Unit, Division of Oral Biosciences, Dublin Dental School and Hospital, Trinity College Dublin, University of Dublin, Dublin 2, Republic of Ireland. gpmoran@dental.tcd.ie.en
dc.identifier.journalEukaryotic cellen
dc.description.provinceLeinster-
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