Comparative transcript profiling of Candida albicans and Candida dubliniensis identifies SFL2, a C. albicans gene required for virulence in a reconstituted epithelial infection model.

Hdl Handle:
http://hdl.handle.net/10147/123302
Title:
Comparative transcript profiling of Candida albicans and Candida dubliniensis identifies SFL2, a C. albicans gene required for virulence in a reconstituted epithelial infection model.
Authors:
Spiering, Martin J; Moran, Gary P; Chauvel, Murielle; Maccallum, Donna M; Higgins, Judy; Hokamp, Karsten; Yeomans, Tim; d'Enfert, Christophe; Coleman, David C; Sullivan, Derek J
Affiliation:
Microbiology Research Unit, Division of Oral Biosciences, Dublin Dental School and Hospital, University of Dublin, Trinity College, Dublin 2, Ireland.
Citation:
Comparative transcript profiling of Candida albicans and Candida dubliniensis identifies SFL2, a C. albicans gene required for virulence in a reconstituted epithelial infection model. 2010, 9 (2):251-65 Eukaryotic Cell
Journal:
Eukaryotic cell
Issue Date:
Feb-2010
URI:
http://hdl.handle.net/10147/123302
DOI:
10.1128/EC.00291-09
PubMed ID:
20023067
Additional Links:
http://ec.asm.org/cgi/content/full/9/2/251
Abstract:
Candida albicans and Candida dubliniensis are closely related species displaying differences in virulence and genome content, therefore providing potential opportunities to identify novel C. albicans virulence genes. C. albicans gene arrays were used for comparative analysis of global gene expression in the two species in reconstituted human oral epithelium (RHE). C. albicans (SC5314) showed upregulation of hypha-specific and virulence genes within 30 min postinoculation, coinciding with rapid induction of filamentation and increased RHE damage. C. dubliniensis (CD36) showed no detectable upregulation of hypha-specific genes, grew as yeast, and caused limited RHE damage. Several genes absent or highly divergent in C. dubliniensis were upregulated in C. albicans. One such gene, SFL2 (orf19.3969), encoding a putative heat shock factor, was deleted in C. albicans. DeltaDeltasfl2 cells failed to filament under a range of hypha-inducing conditions and exhibited greatly reduced RHE damage, reversed by reintroduction of SFL2 into the DeltaDeltasfl2 strain. Moreover, SFL2 overexpression in C. albicans triggered hyphal morphogenesis. Although SFL2 deletion had no apparent effect on host survival in the murine model of systemic infection, DeltaDeltasfl2 strain-infected kidney tissues contained only yeast cells. These results suggest a role for SFL2 in morphogenesis and an indirect role in C. albicans pathogenesis in epithelial tissues.
Language:
en
MeSH:
Animals; Candida; Candida albicans; Candidiasis; Epithelial Cells; Female; Fungal Proteins; Gene Expression Profiling; Gene Expression Regulation, Fungal; Genes, Fungal; Genome, Fungal; Mice; Mice, Inbred BALB C; Models, Animal; Transcription, Genetic; Virulence
ISSN:
1535-9786

Full metadata record

DC FieldValue Language
dc.contributor.authorSpiering, Martin Jen
dc.contributor.authorMoran, Gary Pen
dc.contributor.authorChauvel, Murielleen
dc.contributor.authorMaccallum, Donna Men
dc.contributor.authorHiggins, Judyen
dc.contributor.authorHokamp, Karstenen
dc.contributor.authorYeomans, Timen
dc.contributor.authord'Enfert, Christopheen
dc.contributor.authorColeman, David Cen
dc.contributor.authorSullivan, Derek Jen
dc.date.accessioned2011-03-02T12:48:45Z-
dc.date.available2011-03-02T12:48:45Z-
dc.date.issued2010-02-
dc.identifier.citationComparative transcript profiling of Candida albicans and Candida dubliniensis identifies SFL2, a C. albicans gene required for virulence in a reconstituted epithelial infection model. 2010, 9 (2):251-65 Eukaryotic Cellen
dc.identifier.issn1535-9786-
dc.identifier.pmid20023067-
dc.identifier.doi10.1128/EC.00291-09-
dc.identifier.urihttp://hdl.handle.net/10147/123302-
dc.description.abstractCandida albicans and Candida dubliniensis are closely related species displaying differences in virulence and genome content, therefore providing potential opportunities to identify novel C. albicans virulence genes. C. albicans gene arrays were used for comparative analysis of global gene expression in the two species in reconstituted human oral epithelium (RHE). C. albicans (SC5314) showed upregulation of hypha-specific and virulence genes within 30 min postinoculation, coinciding with rapid induction of filamentation and increased RHE damage. C. dubliniensis (CD36) showed no detectable upregulation of hypha-specific genes, grew as yeast, and caused limited RHE damage. Several genes absent or highly divergent in C. dubliniensis were upregulated in C. albicans. One such gene, SFL2 (orf19.3969), encoding a putative heat shock factor, was deleted in C. albicans. DeltaDeltasfl2 cells failed to filament under a range of hypha-inducing conditions and exhibited greatly reduced RHE damage, reversed by reintroduction of SFL2 into the DeltaDeltasfl2 strain. Moreover, SFL2 overexpression in C. albicans triggered hyphal morphogenesis. Although SFL2 deletion had no apparent effect on host survival in the murine model of systemic infection, DeltaDeltasfl2 strain-infected kidney tissues contained only yeast cells. These results suggest a role for SFL2 in morphogenesis and an indirect role in C. albicans pathogenesis in epithelial tissues.-
dc.language.isoenen
dc.relation.urlhttp://ec.asm.org/cgi/content/full/9/2/251en
dc.subject.meshAnimals-
dc.subject.meshCandida-
dc.subject.meshCandida albicans-
dc.subject.meshCandidiasis-
dc.subject.meshEpithelial Cells-
dc.subject.meshFemale-
dc.subject.meshFungal Proteins-
dc.subject.meshGene Expression Profiling-
dc.subject.meshGene Expression Regulation, Fungal-
dc.subject.meshGenes, Fungal-
dc.subject.meshGenome, Fungal-
dc.subject.meshMice-
dc.subject.meshMice, Inbred BALB C-
dc.subject.meshModels, Animal-
dc.subject.meshTranscription, Genetic-
dc.subject.meshVirulence-
dc.titleComparative transcript profiling of Candida albicans and Candida dubliniensis identifies SFL2, a C. albicans gene required for virulence in a reconstituted epithelial infection model.en
dc.contributor.departmentMicrobiology Research Unit, Division of Oral Biosciences, Dublin Dental School and Hospital, University of Dublin, Trinity College, Dublin 2, Ireland.en
dc.identifier.journalEukaryotic cellen
dc.description.provinceLeinster-
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