Yersinia outer protein YopE affects the actin cytoskeleton in Dictyostelium discoideum through targeting of multiple Rho family GTPases

Hdl Handle:
http://hdl.handle.net/10147/120927
Title:
Yersinia outer protein YopE affects the actin cytoskeleton in Dictyostelium discoideum through targeting of multiple Rho family GTPases
Authors:
Vlahou, Georgia; Schmidt, Oxana; Wagner, Bettina; Uenlue, Handan; Dersch, Petra; Rivero, Francisco; Weissenmayer, Barbara A
Issue Date:
14-Jul-2009
URI:
http://hdl.handle.net/10147/120927
Abstract:
Abstract Background All human pathogenic Yersinia species share a virulence-associated type III secretion system that translocates Yersinia effector proteins into host cells to counteract infection-induced signaling responses and prevent phagocytosis. Dictyostelium discoideum has been recently used to study the effects of bacterial virulence factors produced by internalized pathogens. In this study we explored the potential of Dictyostelium as model organism for analyzing the effects of ectopically expressed Yersinia outer proteins (Yops). Results The Yersinia pseudotuberculosis virulence factors YopE, YopH, YopM and YopJ were expressed de novo within Dictyostelium and their effects on growth in axenic medium and on bacterial lawns were analyzed. No severe effect was observed for YopH, YopJ and YopM, but expression of YopE, which is a GTPase activating protein for Rho GTPases, was found to be highly detrimental. GFP-tagged YopE expressing cells had less conspicuous cortical actin accumulation and decreased amounts of F-actin. The actin polymerization response upon cAMP stimulation was impaired, although chemotaxis was unaffected. YopE also caused reduced uptake of yeast particles. These alterations are probably due to impaired Rac1 activation. We also found that YopE predominantly associates with intracellular membranes including the Golgi apparatus and inhibits the function of moderately overexpressed RacH. Conclusion The phenotype elicited by YopE in Dictyostelium can be explained, at least in part, by inactivation of one or more Rho family GTPases. It further demonstrates that the social amoeba Dictyostelium discoideum can be used as an efficient and easy-to-handle model organism in order to analyze the function of a translocated GAP protein of a human pathogen.
Item Type:
Article
Language:
en
Keywords:
MICROBIOLOGY; RESEARCH PROJECT
ISSN:
http://dx.doi.org/10.1186/1471-2180-9-138

Full metadata record

DC FieldValue Language
dc.contributor.authorVlahou, Georgiaen
dc.contributor.authorSchmidt, Oxanaen
dc.contributor.authorWagner, Bettinaen
dc.contributor.authorUenlue, Handanen
dc.contributor.authorDersch, Petraen
dc.contributor.authorRivero, Franciscoen
dc.contributor.authorWeissenmayer, Barbara Aen
dc.date.accessioned2011-02-02T10:34:39Z-
dc.date.available2011-02-02T10:34:39Z-
dc.date.issued2009-07-14-
dc.identifier.issnhttp://dx.doi.org/10.1186/1471-2180-9-138-
dc.identifier.urihttp://hdl.handle.net/10147/120927-
dc.description.abstractAbstract Background All human pathogenic Yersinia species share a virulence-associated type III secretion system that translocates Yersinia effector proteins into host cells to counteract infection-induced signaling responses and prevent phagocytosis. Dictyostelium discoideum has been recently used to study the effects of bacterial virulence factors produced by internalized pathogens. In this study we explored the potential of Dictyostelium as model organism for analyzing the effects of ectopically expressed Yersinia outer proteins (Yops). Results The Yersinia pseudotuberculosis virulence factors YopE, YopH, YopM and YopJ were expressed de novo within Dictyostelium and their effects on growth in axenic medium and on bacterial lawns were analyzed. No severe effect was observed for YopH, YopJ and YopM, but expression of YopE, which is a GTPase activating protein for Rho GTPases, was found to be highly detrimental. GFP-tagged YopE expressing cells had less conspicuous cortical actin accumulation and decreased amounts of F-actin. The actin polymerization response upon cAMP stimulation was impaired, although chemotaxis was unaffected. YopE also caused reduced uptake of yeast particles. These alterations are probably due to impaired Rac1 activation. We also found that YopE predominantly associates with intracellular membranes including the Golgi apparatus and inhibits the function of moderately overexpressed RacH. Conclusion The phenotype elicited by YopE in Dictyostelium can be explained, at least in part, by inactivation of one or more Rho family GTPases. It further demonstrates that the social amoeba Dictyostelium discoideum can be used as an efficient and easy-to-handle model organism in order to analyze the function of a translocated GAP protein of a human pathogen.-
dc.language.isoenen
dc.subjectMICROBIOLOGYen
dc.subjectRESEARCH PROJECTen
dc.titleYersinia outer protein YopE affects the actin cytoskeleton in Dictyostelium discoideum through targeting of multiple Rho family GTPasesen
dc.typeArticleen
dc.language.rfc3066en-
dc.rights.holderVlahou et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2011-01-05T12:01:35Z-
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