Bifidobacterium animalis AHC7 protects against pathogen-induced NF-kappaB activation in vivo

Hdl Handle:
http://hdl.handle.net/10147/120906
Title:
Bifidobacterium animalis AHC7 protects against pathogen-induced NF-kappaB activation in vivo
Authors:
O'Mahony, David; Murphy, Sharon; Boileau, Thomas; Park, JeanSoon; O'Brien, Frances; Groeger, David; Konieczna, Patrycja; Ziegler, Mario; Scully, Paul; Shanahan, Fergus; Kiely, Barry; O'Mahony, Liam
Issue Date:
22-Dec-2010
URI:
http://hdl.handle.net/10147/120906
Abstract:
Abstract Background Bifidobacteria and lactobacilli are among the early and important colonizers of the gastrointestinal tract and are generally considered to be part of a normal, healthy microbiota. It is believed that specific strains within the microbiota can influence host immune-reactivity and may play a role in protection from infection and aberrant inflammatory activity. One such strain, Bifidobacterium animalis AHC7, has been previously shown to protect against Salmonella typhimurium infection in mice and helps resolve acute idiopathic diarrhea in dogs. The aim of this study was to investigate the potential molecular and cellular mechanisms underpinning the Bifidobacterium animalis AHC7 protective effect. Results Following 4 hours of infection with Salmonella typhimurium, NF-κB activation was significantly elevated in vivo in placebo and Enterococcus faecium-fed animals while Bifidobacterium animalis AHC7 consumption significantly attenuated the NF-κB response. In vitro anti-CD3/CD28 stimulated Peyer's patch cells secreted significantly less TNF-α and IFN-γ following Bifidobacterium animalis AHC7 consumption. Stimulated cells released more IL-12p70 but this difference did not reach statistical significance. No alteration in mucosal IL-6, IL-10 or MCP-1 levels were observed. No statistically significant change in the cytokine profile of mesenteric lymph node cells was noted. In vitro, Bifidobacterium animalis AHC7 was bound by dendritic cells and induced secretion of both IL-10 and IL-12p70. In addition, co-culture of CD4+ T cells with Bifidobacterium animalis AHC7-stimulated dendritic cells resulted in a significant increase in CD25+Foxp3+ T cell numbers. Conclusion Bifidobacterium animalis AHC7 exerts an anti-inflammatory effect via the attenuation of pro-inflammatory transcription factor activation in response to an infectious insult associated with modulation of pro-inflammatory cytokine production within the mucosa. The cellular mechanism underpinning Bifidobacterium animalis AHC7 mediated attenuation of NF-κB activation may include recognition of the bacterium by dendritic cells and induction of CD25+Foxp3+ T cells.
Item Type:
Article
Language:
en
Keywords:
IMMUNE SYSTEM
Local subject classification:
MICROBIOLOGY
ISSN:
http://dx.doi.org/10.1186/1471-2172-11-63

Full metadata record

DC FieldValue Language
dc.contributor.authorO'Mahony, Daviden
dc.contributor.authorMurphy, Sharonen
dc.contributor.authorBoileau, Thomasen
dc.contributor.authorPark, JeanSoonen
dc.contributor.authorO'Brien, Francesen
dc.contributor.authorGroeger, Daviden
dc.contributor.authorKonieczna, Patrycjaen
dc.contributor.authorZiegler, Marioen
dc.contributor.authorScully, Paulen
dc.contributor.authorShanahan, Fergusen
dc.contributor.authorKiely, Barryen
dc.contributor.authorO'Mahony, Liamen
dc.date.accessioned2011-02-01T17:28:30Z-
dc.date.available2011-02-01T17:28:30Z-
dc.date.issued2010-12-22-
dc.identifier.issnhttp://dx.doi.org/10.1186/1471-2172-11-63-
dc.identifier.urihttp://hdl.handle.net/10147/120906-
dc.description.abstractAbstract Background Bifidobacteria and lactobacilli are among the early and important colonizers of the gastrointestinal tract and are generally considered to be part of a normal, healthy microbiota. It is believed that specific strains within the microbiota can influence host immune-reactivity and may play a role in protection from infection and aberrant inflammatory activity. One such strain, Bifidobacterium animalis AHC7, has been previously shown to protect against Salmonella typhimurium infection in mice and helps resolve acute idiopathic diarrhea in dogs. The aim of this study was to investigate the potential molecular and cellular mechanisms underpinning the Bifidobacterium animalis AHC7 protective effect. Results Following 4 hours of infection with Salmonella typhimurium, NF-κB activation was significantly elevated in vivo in placebo and Enterococcus faecium-fed animals while Bifidobacterium animalis AHC7 consumption significantly attenuated the NF-κB response. In vitro anti-CD3/CD28 stimulated Peyer's patch cells secreted significantly less TNF-α and IFN-γ following Bifidobacterium animalis AHC7 consumption. Stimulated cells released more IL-12p70 but this difference did not reach statistical significance. No alteration in mucosal IL-6, IL-10 or MCP-1 levels were observed. No statistically significant change in the cytokine profile of mesenteric lymph node cells was noted. In vitro, Bifidobacterium animalis AHC7 was bound by dendritic cells and induced secretion of both IL-10 and IL-12p70. In addition, co-culture of CD4+ T cells with Bifidobacterium animalis AHC7-stimulated dendritic cells resulted in a significant increase in CD25+Foxp3+ T cell numbers. Conclusion Bifidobacterium animalis AHC7 exerts an anti-inflammatory effect via the attenuation of pro-inflammatory transcription factor activation in response to an infectious insult associated with modulation of pro-inflammatory cytokine production within the mucosa. The cellular mechanism underpinning Bifidobacterium animalis AHC7 mediated attenuation of NF-κB activation may include recognition of the bacterium by dendritic cells and induction of CD25+Foxp3+ T cells.-
dc.language.isoenen
dc.subjectIMMUNE SYSTEMen
dc.subject.otherMICROBIOLOGYen
dc.titleBifidobacterium animalis AHC7 protects against pathogen-induced NF-kappaB activation in vivoen
dc.typeArticleen
dc.language.rfc3066en-
dc.rights.holderO'Mahony et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2011-01-05T16:01:24Z-
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