The dissemination of C10 cysteine protease genes in Bacteroides fragilis by mobile genetic elements

Hdl Handle:
http://hdl.handle.net/10147/119131
Title:
The dissemination of C10 cysteine protease genes in Bacteroides fragilis by mobile genetic elements
Authors:
Thornton, Roibeard F; Kagawa, Todd F; O'Toole, Paul W; Cooney, Jakki C
Citation:
BMC Microbiology. 2010 Apr 23;10(1):122
Issue Date:
23-Apr-2010
URI:
http://hdl.handle.net/10147/119131
Abstract:
Abstract Background The C10 family of cysteine proteases includes enzymes that contribute to the virulence of bacterial pathogens, such as SpeB in Streptococcus pyogenes. The presence of homologues of cysteine protease genes in human commensal organisms has not been examined. Bacteroides fragilis is a member of the dominant Bacteroidetes phylum of the human intestinal microbiota, and is a significant opportunistic pathogen. Results Four homologues of the streptococcal virulence factor SpeB were identified in the B. fragilis genome. These four protease genes, two were directly contiguous to open reading frames predicted to encode staphostatin-like inhibitors, with which the protease genes were co-transcribed. Two of these protease genes are unique to B. fragilis 638R and are associated with two large genomic insertions. Gene annotation indicated that one of these insertions was a conjugative Tn-like element and the other was a prophage-like element, which was shown to be capable of excision. Homologues of the B. fragilis C10 protease genes were present in a panel of clinical isolates, and in DNA extracted from normal human faecal microbiota. Conclusions This study suggests a mechanism for the evolution and dissemination of an important class of protease in major members of the normal human microbiota.
Item Type:
Journal Article

Full metadata record

DC FieldValue Language
dc.contributor.authorThornton, Roibeard F-
dc.contributor.authorKagawa, Todd F-
dc.contributor.authorO'Toole, Paul W-
dc.contributor.authorCooney, Jakki C-
dc.date.accessioned2011-01-11T12:41:28Z-
dc.date.available2011-01-11T12:41:28Z-
dc.date.issued2010-04-23-
dc.identifierhttp://dx.doi.org/10.1186/1471-2180-10-122-
dc.identifier.citationBMC Microbiology. 2010 Apr 23;10(1):122-
dc.identifier.urihttp://hdl.handle.net/10147/119131-
dc.description.abstractAbstract Background The C10 family of cysteine proteases includes enzymes that contribute to the virulence of bacterial pathogens, such as SpeB in Streptococcus pyogenes. The presence of homologues of cysteine protease genes in human commensal organisms has not been examined. Bacteroides fragilis is a member of the dominant Bacteroidetes phylum of the human intestinal microbiota, and is a significant opportunistic pathogen. Results Four homologues of the streptococcal virulence factor SpeB were identified in the B. fragilis genome. These four protease genes, two were directly contiguous to open reading frames predicted to encode staphostatin-like inhibitors, with which the protease genes were co-transcribed. Two of these protease genes are unique to B. fragilis 638R and are associated with two large genomic insertions. Gene annotation indicated that one of these insertions was a conjugative Tn-like element and the other was a prophage-like element, which was shown to be capable of excision. Homologues of the B. fragilis C10 protease genes were present in a panel of clinical isolates, and in DNA extracted from normal human faecal microbiota. Conclusions This study suggests a mechanism for the evolution and dissemination of an important class of protease in major members of the normal human microbiota.-
dc.titleThe dissemination of C10 cysteine protease genes in Bacteroides fragilis by mobile genetic elements-
dc.typeJournal Article-
dc.language.rfc3066en-
dc.rights.holderThornton et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2010-12-15T21:04:24Z-
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