In silico analysis highlights the frequency and diversity of type 1 lantibiotic gene clusters in genome sequenced bacteria

Hdl Handle:
http://hdl.handle.net/10147/118010
Title:
In silico analysis highlights the frequency and diversity of type 1 lantibiotic gene clusters in genome sequenced bacteria
Authors:
Marsh, Alan J; O'Sullivan, Orla; Ross, R PAUL; Cotter, Paul D; Hill, Colin
Citation:
BMC Genomics. 2010 Nov 30;11(1):679
Issue Date:
30-Nov-2010
URI:
http://hdl.handle.net/10147/118010
Abstract:
Abstract Background Lantibiotics are lanthionine-containing, post-translationally modified antimicrobial peptides. These peptides have significant, but largely untapped, potential as preservatives and chemotherapeutic agents. Type 1 lantibiotics are those in which lanthionine residues are introduced into the structural peptide (LanA) through the activity of separate lanthionine dehydratase (LanB) and lanthionine synthetase (LanC) enzymes. Here we take advantage of the conserved nature of LanC enzymes to devise an in silico approach to identify potential lantibiotic-encoding gene clusters in genome sequenced bacteria. Results In total 49 novel type 1 lantibiotic clusters were identified which unexpectedly were associated with species, genera and even phyla of bacteria which have not previously been associated with lantibiotic production. Conclusions Multiple type 1 lantibiotic gene clusters were identified at a frequency that suggests that these antimicrobials are much more widespread than previously thought. These clusters represent a rich repository which can yield a large number of valuable novel antimicrobials and biosynthetic enzymes.
Item Type:
Journal Article

Full metadata record

DC FieldValue Language
dc.contributor.authorMarsh, Alan J-
dc.contributor.authorO'Sullivan, Orla-
dc.contributor.authorRoss, R PAUL-
dc.contributor.authorCotter, Paul D-
dc.contributor.authorHill, Colin-
dc.date.accessioned2010-12-17T12:40:33Z-
dc.date.available2010-12-17T12:40:33Z-
dc.date.issued2010-11-30-
dc.identifierhttp://dx.doi.org/10.1186/1471-2164-11-679-
dc.identifier.citationBMC Genomics. 2010 Nov 30;11(1):679-
dc.identifier.urihttp://hdl.handle.net/10147/118010-
dc.description.abstractAbstract Background Lantibiotics are lanthionine-containing, post-translationally modified antimicrobial peptides. These peptides have significant, but largely untapped, potential as preservatives and chemotherapeutic agents. Type 1 lantibiotics are those in which lanthionine residues are introduced into the structural peptide (LanA) through the activity of separate lanthionine dehydratase (LanB) and lanthionine synthetase (LanC) enzymes. Here we take advantage of the conserved nature of LanC enzymes to devise an in silico approach to identify potential lantibiotic-encoding gene clusters in genome sequenced bacteria. Results In total 49 novel type 1 lantibiotic clusters were identified which unexpectedly were associated with species, genera and even phyla of bacteria which have not previously been associated with lantibiotic production. Conclusions Multiple type 1 lantibiotic gene clusters were identified at a frequency that suggests that these antimicrobials are much more widespread than previously thought. These clusters represent a rich repository which can yield a large number of valuable novel antimicrobials and biosynthetic enzymes.-
dc.titleIn silico analysis highlights the frequency and diversity of type 1 lantibiotic gene clusters in genome sequenced bacteria-
dc.typeJournal Article-
dc.language.rfc3066en-
dc.rights.holderMarsh et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2010-12-15T12:04:24Z-
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