MYEOV (myeloma overexpressed gene) drives colon cancer cell migration and is regulated by PGE2.

Hdl Handle:
http://hdl.handle.net/10147/115119
Title:
MYEOV (myeloma overexpressed gene) drives colon cancer cell migration and is regulated by PGE2.
Authors:
Lawlor, Garrett; Doran, Peter P; MacMathuna, Padraic; Murray, David W
Affiliation:
Gastrointestinal Unit, Mater Misericordiae University Hospital, Eccles Street, Dublin 7, Ireland.
Citation:
MYEOV (myeloma overexpressed gene) drives colon cancer cell migration and is regulated by PGE2. 2010, 29:81 J. Exp. Clin. Cancer Res.
Journal:
Journal of experimental & clinical cancer research : CR
Issue Date:
2010
URI:
http://hdl.handle.net/10147/115119
DOI:
10.1186/1756-9966-29-81
PubMed ID:
20569498
Abstract:
INTRODUCTION: We have previously reported that Myeov (MYEloma OVerexpressed gene) expression is enhanced in colorectal cancer (CRC) and that it promotes CRC cell proliferation and invasion. The role of Myeov in CRC migration is unclear. ProstaglandinE2 (PGE 2) is a known factor in promoting CRC carcinogenesis. The role of PGE 2 in modulating Myeov expression has also not been defined. AIM: To assess the role of Myeov expression in CRC cell migration and to evaluate the role of PGE 2 in Myeov bioactivity. METHODS: siRNA mediated Myeov knockdown was achieved in T84 CRC cells. Knockdown was assessed using quantitative real time PCR. The effect of knockdown on CRC cell migration was assessed using a scratch wound healing assay. Separately, T84 cells were treated with PGE 2 (0.00025 micro M, 0.1 micro M and 1 micro M) from 30 min to 3 hours and the effect on Myeov gene expression was assessed using real time PCR. RESULTS: Myeov knockdown resulted in a significant reduction in CRC cell migration, observable as early as 12 hours (P < 0.05) with a 39% reduction compared to control at 36 hours (p < 0.01). Myeov expression was enhanced after treatment with PGE 2, with the greatest effect seen at 60 mins for all 3 PGE 2 doses. This response was dose dependent with a 290%, 550% & 1,000% increase in Myeov expression for 0.00025 micro M, 0.1 micro M and 1 micro M PGE 2 respectively. CONCLUSION: In addition to promoting CRC proliferation and invasion, our findings indicate that Myeov stimulates CRC cell migration, and its expression may be PGE 2 dependant.
Item Type:
Article
Language:
en
Keywords:
INTESTINAL CANCER; GENETICS
MeSH:
Cell Line, Tumor; Cell Movement; Cell Proliferation; Colonic Neoplasms; Dinoprostone; Humans; Proto-Oncogene Proteins; RNA, Messenger; RNA, Small Interfering; Reverse Transcriptase Polymerase Chain Reaction
ISSN:
1756-9966

Full metadata record

DC FieldValue Language
dc.contributor.authorLawlor, Garretten
dc.contributor.authorDoran, Peter Pen
dc.contributor.authorMacMathuna, Padraicen
dc.contributor.authorMurray, David Wen
dc.date.accessioned2010-11-09T12:39:47Z-
dc.date.available2010-11-09T12:39:47Z-
dc.date.issued2010-
dc.identifier.citationMYEOV (myeloma overexpressed gene) drives colon cancer cell migration and is regulated by PGE2. 2010, 29:81 J. Exp. Clin. Cancer Res.en
dc.identifier.issn1756-9966-
dc.identifier.pmid20569498-
dc.identifier.doi10.1186/1756-9966-29-81-
dc.identifier.urihttp://hdl.handle.net/10147/115119-
dc.description.abstractINTRODUCTION: We have previously reported that Myeov (MYEloma OVerexpressed gene) expression is enhanced in colorectal cancer (CRC) and that it promotes CRC cell proliferation and invasion. The role of Myeov in CRC migration is unclear. ProstaglandinE2 (PGE 2) is a known factor in promoting CRC carcinogenesis. The role of PGE 2 in modulating Myeov expression has also not been defined. AIM: To assess the role of Myeov expression in CRC cell migration and to evaluate the role of PGE 2 in Myeov bioactivity. METHODS: siRNA mediated Myeov knockdown was achieved in T84 CRC cells. Knockdown was assessed using quantitative real time PCR. The effect of knockdown on CRC cell migration was assessed using a scratch wound healing assay. Separately, T84 cells were treated with PGE 2 (0.00025 micro M, 0.1 micro M and 1 micro M) from 30 min to 3 hours and the effect on Myeov gene expression was assessed using real time PCR. RESULTS: Myeov knockdown resulted in a significant reduction in CRC cell migration, observable as early as 12 hours (P < 0.05) with a 39% reduction compared to control at 36 hours (p < 0.01). Myeov expression was enhanced after treatment with PGE 2, with the greatest effect seen at 60 mins for all 3 PGE 2 doses. This response was dose dependent with a 290%, 550% & 1,000% increase in Myeov expression for 0.00025 micro M, 0.1 micro M and 1 micro M PGE 2 respectively. CONCLUSION: In addition to promoting CRC proliferation and invasion, our findings indicate that Myeov stimulates CRC cell migration, and its expression may be PGE 2 dependant.-
dc.language.isoenen
dc.subjectINTESTINAL CANCERen
dc.subjectGENETICSen
dc.subject.meshCell Line, Tumor-
dc.subject.meshCell Movement-
dc.subject.meshCell Proliferation-
dc.subject.meshColonic Neoplasms-
dc.subject.meshDinoprostone-
dc.subject.meshHumans-
dc.subject.meshProto-Oncogene Proteins-
dc.subject.meshRNA, Messenger-
dc.subject.meshRNA, Small Interfering-
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction-
dc.titleMYEOV (myeloma overexpressed gene) drives colon cancer cell migration and is regulated by PGE2.en
dc.typeArticleen
dc.contributor.departmentGastrointestinal Unit, Mater Misericordiae University Hospital, Eccles Street, Dublin 7, Ireland.en
dc.identifier.journalJournal of experimental & clinical cancer research : CRen

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